Phytochemical study of the headspace volatile organic compounds of fresh algae and seagrass from the Adriatic Sea (single point collection)

Igor Jerković; Zvonimir Marijanović; Marin Roje; Piotr M Kuś; Stela Jokić; Rozelinda Čož-Rakovac

doi:10.1371/journal.pone.0196462

Phytochemical study of the headspace volatile organic compounds of fresh algae and seagrass from the Adriatic Sea (single point collection)

PLoS One. 2018 May 8;13(5):e0196462. doi: 10.1371/journal.pone.0196462. eCollection 2018.

Authors

Igor Jerković¹, Zvonimir Marijanović², Marin Roje³, Piotr M Kuś⁴, Stela Jokić⁵, Rozelinda Čož-Rakovac⁶

Affiliations

¹ Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, HR Split, Croatia.
² Department of Food Technology and Biotechnology, Faculty of Chemistry and Technology, University of Split, HR Split, Croatia.
³ Division of Organic Chemistry and Biochemistry, Ruđer Bošković Institute, HR Zagreb, Croatia.
⁴ Department of Pharmacognosy, Wrocław Medical University, Wrocław, Poland.
⁵ Department of Process Engineering, Faculty of Food Technology, Josip Juraj Strossmayer University of Osijek, HR Osijek, Croatia.
⁶ Laboratory for Aquaculture Biotechnology, Ruđer Bošković Institute, HR Zagreb, Croatia.

Abstract

Performed phytochemical study contributes to the knowledge of volatile organic compounds (VOCs) of Halopteris filicina (Grateloup) Kützing, Dictyota dichotoma (Hudson) J. V. Lamouroux, Posidonia oceanica (L.) Delile and Flabellia petiolata (Turra) Nizamuddin from the Adriatic Sea (single point collection). VOCs were investigated by headspace solid-phase microextraction (HS-SPME) and analysed by gas chromatography and mass spectrometry (GC-MS/FID). H. filicina headspace contained dimethyl sulfide (DMS; 12.8%), C8-compounds (e.g. fucoserratene (I; 9.5%)), benzaldehyde (II; 8.7%), alkane C17, dictyopterene D and C (III, IV), tribromomethane (V), 1-iodopentane, others. F. petiolata headspace was characterized by DMS (22.2%), 6-methylhept-5-en-2-one (9.5%), C17 (9.1%), II (6.5%), compounds I-V. DMS (59.3%), C15 (14.5%), C17 (7.2%) and C19 (6.3%) dominated in P. oceanica headspace. Sesquiterpenes were found in D. dichotoma, predominantly germacrene D (28.3%) followed by other cadinenyl (abundant), muurolenyl and amorphenyl structures. Determined VOCs may be significant for chemosystematics and chemical communications in marine ecosystem.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alismatales / chemistry
Aquatic Organisms / chemistry*
Chlorophyta / chemistry
Gas Chromatography-Mass Spectrometry / methods
Mediterranean Sea
Phaeophyceae / chemistry
Phytochemicals / analysis*
Solid Phase Microextraction / methods
Volatile Organic Compounds / analysis*

Substances

Phytochemicals
Volatile Organic Compounds

Grants and funding

This research was supported by the Croatian Science Foundation under the project HRZZ-IP-11-2013-8547 (Research of Natural Products and Flavours: Chemical Fingerprinting and Unlocking the Potential) to Igor Jerković and by the Croatian Government and the European Union through the European Regional Development Fund - the Competitiveness and Cohesion Operational Programme (KK.01.1.1.01) – The Scientific Centre of Excellence for Marine Bioprospecting -BioProCro to Rozelinda Čož Rakovac.