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Front Immunol. 2018 Apr 20;9:696. doi: 10.3389/fimmu.2018.00696. eCollection 2018.

Macrophage Lamin A/C Regulates Inflammation and the Development of Obesity-Induced Insulin Resistance.

Author information

1
Soonchunhyang Institute of Medi-Bio Science (SIMS), Soonchunhyang University, Cheon-an, South Korea.
2
Department of Surgery, Soonchunhyang University Hospital, Seoul, South Korea.
3
Department of Plastic and Reconstructive Surgery, Soonchunhyang University Hospital, Gumi, South Korea.
4
Department of Surgery, Soonchunhyang University Gumi Hospital, Gumi, South Korea.
5
The Joslin Diabetes Center, Department of Medicine, Harvard Medical School, Boston, MA, United States.

Abstract

Obesity-induced chronic low-grade inflammation, in particular in adipose tissue, contributes to the development of insulin resistance and type 2 diabetes. However, the mechanism by which obesity induces adipose tissue inflammation has not been completely elucidated. Recent studies suggest that alteration of the nuclear lamina is associated with age-associated chronic inflammation in humans and fly. These findings led us to investigate whether the nuclear lamina regulates obesity-mediated chronic inflammation. In this study, we show that lamin A/C mediates inflammation in macrophages. The gene and protein expression levels of lamin A/C are significantly increased in epididymal adipose tissues from obese rodent models and omental fat from obese human subjects compared to their lean controls. Flow cytometry and gene expression analyses reveal that the protein and gene expression levels of lamin A/C are increased in adipose tissue macrophages (ATMs) by obesity. We further show that ectopic overexpression of lamin A/C in macrophages spontaneously activates NF-κB, and increases the gene expression levels of proinflammatory genes, such as Il6, Tnf, Ccl2, and Nos2. Conversely, deletion of lamin A/C in macrophages reduces LPS-induced expression of these proinflammatory genes. Importantly, we find that myeloid cell-specific lamin A/C deficiency ameliorates obesity-induced insulin resistance and adipose tissue inflammation. Thus, our data suggest that lamin A/C mediates the activation of ATM inflammation by regulating NF-κB, thereby contributing to the development of obesity-induced insulin resistance.

KEYWORDS:

adipose tissue; inflammation; insulin resistance; lamin A/C; macrophages; obesity

PMID:
29731750
PMCID:
PMC5920030
DOI:
10.3389/fimmu.2018.00696
[Indexed for MEDLINE]
Free PMC Article

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