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Curr Biol. 2018 May 21;28(10):1620-1627.e5. doi: 10.1016/j.cub.2018.03.067. Epub 2018 May 3.

Discovery of the First Germline-Restricted Gene by Subtractive Transcriptomic Analysis in the Zebra Finch, Taeniopygia guttata.

Author information

1
Department of Biology, American University, 4400 Massachusetts Avenue NW, Washington, DC 20016, USA.
2
Department of Biology, American University, 4400 Massachusetts Avenue NW, Washington, DC 20016, USA. Electronic address: jbracht@american.edu.

Abstract

Developmentally programmed genome rearrangements are rare in vertebrates, but have been reported in scattered lineages including the bandicoot, hagfish, lamprey, and zebra finch (Taeniopygia guttata) [1]. In the finch, a well-studied animal model for neuroendocrinology and vocal learning [2], one such programmed genome rearrangement involves a germline-restricted chromosome, or GRC, which is found in germlines of both sexes but eliminated from mature sperm [3, 4]. Transmitted only through the oocyte, it displays uniparental female-driven inheritance, and early in embryonic development is apparently eliminated from all somatic tissue in both sexes [3, 4]. The GRC comprises the longest finch chromosome at over 120 million base pairs [3], and previously the only known GRC-derived sequence was repetitive and non-coding [5]. Because the zebra finch genome project was sourced from male muscle (somatic) tissue [6], the remaining genomic sequence and protein-coding content of the GRC remain unknown. Here we report the first protein-coding gene from the GRC: a member of the α-soluble N-ethylmaleimide sensitive fusion protein (NSF) attachment protein (α-SNAP) family hitherto missing from zebra finch gene annotations. In addition to the GRC-encoded α-SNAP, we find an additional paralogous α-SNAP residing in the somatic genome (a somatolog)-making the zebra finch the first example in which α-SNAP is not a single-copy gene. We show divergent, sex-biased expression for the paralogs and also that positive selection is detectable across the bird α-SNAP lineage, including the GRC-encoded α-SNAP. This study presents the identification and evolutionary characterization of the first protein-coding GRC gene in any organism.

KEYWORDS:

Germline-restricted chromosome; RNA-seq; genomics; next-generation sequencing and assembly; phylogenomics; soluble NSF attachment; zebra finch

PMID:
29731307
PMCID:
PMC5977399
DOI:
10.1016/j.cub.2018.03.067
[Indexed for MEDLINE]
Free PMC Article

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