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Mult Scler Relat Disord. 2018 May;22:153-156. doi: 10.1016/j.msard.2018.04.005. Epub 2018 Apr 9.

Dysregulation of NRXN1 by mutant MIR8485 leads to calcium overload in pre-synapses inducing neurodegeneration in Multiple sclerosis.

Author information

1
Laboratory of Genomic Sciences, Department of Studies in Genetics and Genomics, University of Mysore, Manasagangotri, Mysuru 570006, Karnataka, India. Electronic address: yogita@genetics.unimysore.ac.in.
2
Laboratory of Genomic Sciences, Department of Studies in Genetics and Genomics, University of Mysore, Manasagangotri, Mysuru 570006, Karnataka, India. Electronic address: avinash@genetics.uni-mysore.ac.in.

Abstract

OBJECTIVES:

To identify Damaging mutations in microRNAs (miRNAs) and 3' untranslated regions (UTRs) of target genes to establish Multiple sclerosis (MS) disease pathway.

METHODS:

Female aged 16, with Relapsing Remitting Multiple sclerosis (RRMS) was reported with initial symptoms of blurred vision, severe immobility, upper and lower limb numbness and backache. Whole Exome Sequencing (WES) and disease pathway analysis was performed to identify mutations in miRNAs and UTRs.

RESULTS:

We identified Deleterious/Damaging multibase mutations in MIR8485 and NRXN1. miR-8485 was found carrying frameshift homozygous deletion of bases CA, while NRXN1 was found carrying nonframeshift homozygous substitution of bases CT to TC in exon 8 replacing Serine with Leucine.

CONCLUSIONS:

Mutations in miR-8485 and NRXN1 was found to alter calcium homeostasis and NRXN1/NLGN1 cell adhesion molecule binding affinities. The miR-8485 mutation leads to overexpression of NRXN1 altering pre-synaptic Ca2+ homeostasis, inducing neurodegeneration.

KEYWORDS:

Calcium homeostasis; Multiple sclerosis; NRXN1; Neurodegeneration; UTRs; miR-8485

PMID:
29729524
DOI:
10.1016/j.msard.2018.04.005
[Indexed for MEDLINE]

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