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Plant Biotechnol J. 2018 Dec;16(12):2063-2076. doi: 10.1111/pbi.12939. Epub 2018 May 24.

Mitochondrial alternative oxidase-dependent autophagy involved in ethylene-mediated drought tolerance in Solanum lycopersicum.

Author information

1
Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, Sichuan, China.
2
Life Science and Technology College and Ecological Security and Protection Key Laboratory of Sichuan Province, Mianyang Normal University, Mianyang, China.
3
Life Science and Biotechnology, Wuhan Bioengineering Institute, Wuhan, China.

Abstract

Mitochondrial alternative oxidase (AOX) is involved in a large number of plant physiological processes, such as growth, development and stress responses; however, the exact role of AOX in response to drought remains unclear. In our study, we provide solid evidences that the activated AOX capacity positively involved in ethylene-induced drought tolerance, in tomato (Solanum lycopersicum), accompanied by the changing level of hydrogen peroxide (H2 O2 ) and autophagy. In AOX1a-RNAi plants, the ethylene-induced drought tolerance was aggravated and associated with decreasing level of autophagy. The H2 O2 level was relatively higher in AOX1a-RNAi plants, whereas it was lower in AOX1a-overexpressing (35S-AOX1a-OE) plants after 1-(aminocarbonyl)-1-cyclopropanecarboxylic acid (ACC) pretreatment in the 14th day under drought stress. Interestingly, the accumulation of autophagosome was accompanied by the changing level of reactive oxygen species (ROS) in AOX transgenic tomato under drought stress whether or not pretreated with ACC. Pharmacological scavenging of H2 O2 accumulation in AOX1a-RNAi (aox19) stimulated autophagy acceleration under drought stress, and it seems that AOX-dependent ROS signalling is critical in triggering autophagy. Lower levels of ROS signalling positively induce autophagy activity, whereas higher ROS level would lead to rapid programmed cell death (PCD), especially in ethylene-mediated drought tolerance. Moreover, ethylene-induced autophagy during drought stress also can be through ERF5 binding to the promoters of ATG8d and ATG18h. These results demonstrated that AOX plays an essential role in ethylene-induced drought tolerance and also played important roles in mediating autophagy generation via balancing ROS level.

KEYWORDS:

Solanum lycopersicum ; alternative oxidase; autophagy; drought; ethylene; reactive oxygen species

PMID:
29729068
PMCID:
PMC6230944
DOI:
10.1111/pbi.12939
[Indexed for MEDLINE]
Free PMC Article

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