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Int J Pediatr Otorhinolaryngol. 2018 Jun;109:67-71. doi: 10.1016/j.ijporl.2018.03.028. Epub 2018 Mar 28.

Effect of electronic cigarettes on human middle ear.

Author information

1
Department of Otolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, South Korea.
2
Department of Medical Genetics, Hallym University College of Medicine, Chuncheon, South Korea.
3
Department of Otolaryngology-Head and Neck Surgery, Hallym University College of Medicine, Seoul, South Korea.
4
Department of Otolaryngology-Head and Neck Surgery, Hallym University College of Medicine, Seoul, South Korea. Electronic address: brune77@naver.com.

Abstract

OBJECTIVE:

Electronic cigarettes (e-cigarettes) are the most commonly used electronic nicotine delivery systems and are a relatively new product designed for smoking cessation. The market scale of electronic cigarettes is growing rapidly, but the potential impact of e-cigarettes on public health has not yet been verified. In this study, we examined the effect of e-liquids on a human middle ear epithelial cell (HMEEC) line.

MATERIAL AND METHODS:

The main components of e-liquids are propylene glycol, vegetable glycerin and flavoring agents with or without nicotine. We analyzed 73 bottles of e-liquids from 12 different manufacturers, evaluated the trace elements in e-liquids, and identified the cytotoxicity of e-liquids on HMEECs in the presence or absence of nicotine.

RESULTS:

In the trace elements analysis, nickel, arsenic, cadmium, and lead were detected in the e-liquids. E-liquids without nicotine decreased cell viability, and the average IC 50 value of total e-liquids (n = 73) was 2.48 ± 0.93%. Among the different flavors, menthol-flavored e-liquids significantly reduced cell viability, and their average IC 50 value (n = 28) was 1.85 ± 0.80%. The average IC 50 values were distinct among manufacturers and the proportion of the solvents.

CONCLUSION:

The present study provides evidence that e-cigarettes influence and reduce human middle ear cell viability even without the application of nicotine. Additionally, the cytotoxicity of e-liquids was affected by the flavoring agents.

KEYWORDS:

Cytotoxicity; Electronic cigarette; Heavy metals; Human middle ear epithelial cells; Otitis media

PMID:
29728187
DOI:
10.1016/j.ijporl.2018.03.028
[Indexed for MEDLINE]

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