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Aquat Toxicol. 2018 Jul;200:50-61. doi: 10.1016/j.aquatox.2018.04.003. Epub 2018 Apr 18.

Bifenthrin causes transcriptomic alterations in mTOR and ryanodine receptor-dependent signaling and delayed hyperactivity in developing zebrafish (Danio rerio).

Author information

1
Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA; Aquatic Systems Biology, Department of Ecology and Ecosystem Management, Technical University of Munich, Mühlenweg 22, D-85354 Freising, Germany.
2
Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
3
Department of Public Health Sciences, Division of Biostatistics, University of California, Davis, CA 95616, USA.
4
Biology & Marine Biology, University of North Carolina, Wilmington, NC 28403, USA.
5
Aquatic Systems Biology, Department of Ecology and Ecosystem Management, Technical University of Munich, Mühlenweg 22, D-85354 Freising, Germany.
6
Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
7
Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, CA 95616, USA. Electronic address: pjlein@ucdavis.edu.

Abstract

Over the last few decades, the pyrethroid insecticide bifenthrin has been increasingly employed for pest control in urban and agricultural areas, putting humans and wildlife at increased risk of exposure. Exposures to nanomolar (nM) concentrations of bifenthrin have recently been reported to alter calcium oscillations in rodent neurons. Neuronal calcium oscillations are influenced by ryanodine receptor (RyR) activity, which modulates calcium-dependent signaling cascades, including the mechanistic target of rapamycin (mTOR) signaling pathway. RyR activity and mTOR signaling play critical roles in regulating neurodevelopmental processes. However, whether environmentally relevant levels of bifenthrin alter RyR or mTOR signaling pathways to influence neurodevelopment has not been addressed. Therefore, our main objectives in this study were to examine the transcriptomic responses of genes involved in RyR and mTOR signaling pathways in zebrafish (Danio rerio) exposed to low (ng/L) concentrations of bifenthrin, and to assess the potential functional consequences by measuring locomotor responses to external stimuli. Wildtype zebrafish were exposed for 1, 3 and 5 days to 1, 10 and 50 ng/L bifenthrin, followed by a 14 d recovery period. Bifenthrin elicited significant concentration-dependent transcriptional responses in the majority of genes examined in both signaling cascades, and at all time points examined during the acute exposure period (1, 3, and 5 days post fertilization; dpf), and at the post recovery assessment time point (19 dpf). Changes in locomotor behavior were not evident during the acute exposure period, but were observed at 19 dpf, with main effects (increased locomotor behavior) detected in fish exposed developmentally to bifenthrin at 1 or 10 ng/L, but not 50 ng/L. These findings illustrate significant influences of developmental exposures to low (ng/L) concentrations of bifenthrin on neurodevelopmental processes in zebrafish.

KEYWORDS:

Ca2+-dependent signaling; Fish behavior; Insecticide; Neurodevelopment; Pesticide; Pyrethroid

PMID:
29727771
PMCID:
PMC5992106
DOI:
10.1016/j.aquatox.2018.04.003
[Indexed for MEDLINE]
Free PMC Article

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