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Monoclon Antib Immunodiagn Immunother. 2019 Jun;38(3):101-103. doi: 10.1089/mab.2018.0007. Epub 2018 May 3.

Elucidation of the TMab-6 Monoclonal Antibody Epitope Against Telomerase Reverse Transcriptase.

Author information

1
1 Department of Antibody Drug Development, Tohoku University Graduate School of Medicine, Sendai, Japan.
2
2 Department of Oral and Maxillofacial Surgery, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
3
3 Department of Pathology and Laboratory Medicine, Sendai Medical Center, Sendai, Japan.
4
4 New Industry Creation Hatchery Center, Tohoku University, Sendai, Japan.

Abstract

Telomerase reverse transcriptase (TERT) and mutations of the TERT promoter are significant in the pathogenesis of 1p/19q-codeleted oligodendrogliomas and isocitrate dehydrogenase gene wild-type glioblastomas, as well as melanomas and squamous cell carcinomas. We previously developed an antihuman TERT monoclonal antibody (mAb), TMab-6, which is applicable in immunohistochemistry for human tissues. However, the binding epitope of TMab-6 against TERT is yet to be elucidated. In this study, enzyme-linked immunosorbent assay and immunohistochemistry were utilized for investigating the epitope of TMab-6. The findings revealed that the critical epitope of TMab-6 is the TERT sequence PSTSRPPRPWD; Thr310 and Ser311 of TERT are especially significant amino acids for TMab-6 recognition.

KEYWORDS:

TERT; epitope mapping; monoclonal antibody

PMID:
29723111
DOI:
10.1089/mab.2018.0007

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