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Int Immunol. 2018 Jun 26;30(7):301-309. doi: 10.1093/intimm/dxy030.

Mice lacking all of the Skint family genes.

Author information

1
Department of Immunology, Graduate School of Medicine and Faculty of Medicine, University of Tokyo, Hongo, Bunkyo-ku, Tokyo, Japan.
2
Department of Laboratory Animal Medicine, Research Institute, National Center for Global Health and Medicine, Toyama, Shinjuku-ku, Tokyo, Japan.
3
Section of Animal Models, Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Toyama, Shinjuku-ku, Tokyo, Japan.

Abstract

γδT cells develop in the thymus and play important roles in protection against infection and tumor development, but the mechanisms by which the thymic microenvironment supports γδT cell differentiation remain largely unclear. Skint1, a B7-related protein expressed in thymic epithelial cells, was shown to be essential for the development of mouse Vγ5Vδ1 γδT cells. The Skint family in mouse consists of 11 members, Skint1-11. Here we generated mutant mice lacking the entire genomic region that contains all of the Skint genes. These mice exhibited a marked reduction of Vγ5Vδ1 γδT cells in the thymus and skin, but surprisingly, had normal development of other γδT cell subsets and leukocytes including αβT, B and myeloid cells. This phenotype is essentially identical to that of Skint1-deficient mice. These results indicate that the Skint family exerts an exclusive function in regulating the development of Vγ5Vδ1 γδT cells and is dispensable for development of other leukocytes.

PMID:
29718261
DOI:
10.1093/intimm/dxy030

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