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BMC Genomics. 2018 May 2;19(1):311. doi: 10.1186/s12864-018-4692-z.

A genome-wide assessment of conserved SNP alleles reveals a panel of regulatory SNPs relevant to the peripheral nerve.

Author information

1
Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI, USA.
2
Neuroscience Graduate Program, University of Michigan Medical School, Ann Arbor, MI, USA.
3
Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI, USA. antonell@umich.edu.
4
Neuroscience Graduate Program, University of Michigan Medical School, Ann Arbor, MI, USA. antonell@umich.edu.
5
Department of Neurology, University of Michigan Medical School, 3710A Medical Sciences II, 1241 E. Catherine St. SPC 5618, Ann Arbor, MI, 48109, USA. antonell@umich.edu.

Abstract

BACKGROUND:

Identifying functional non-coding variation is critical for defining the genetic contributions to human disease. While single-nucleotide polymorphisms (SNPs) within cis-acting transcriptional regulatory elements have been implicated in disease pathogenesis, not all cell types have been assessed and functional validations have been limited. In particular, the cells of the peripheral nervous system have been excluded from genome-wide efforts to link non-coding SNPs to altered gene function. Addressing this gap is essential for defining the genetic architecture of diseases that affect the peripheral nerve. We developed a computational pipeline to identify SNPs that affect regulatory function (rSNPs) and evaluated our predictions on a set of 144 regions in Schwann cells, motor neurons, and muscle cells.

RESULTS:

We identified 28 regions that display regulatory activity in at least one cell type and 13 SNPs that affect regulatory function. We then tailored our pipeline to one peripheral nerve cell type by incorporating SOX10 ChIP-Seq data; SOX10 is essential for Schwann cells. We prioritized 22 putative SOX10 response elements harboring a SNP and rapidly validated two rSNPs. We then selected one of these elements for further characterization to assess the biological relevance of our approach. Deletion of the element from the genome of cultured Schwann cells-followed by differential gene expression studies-revealed Tubb2b as a candidate target gene. Studying the enhancer in developing mouse embryos revealed activity in SOX10-positive cells including the dorsal root ganglia and melanoblasts.

CONCLUSIONS:

Our efforts provide insight into the utility of employing strict conservation for rSNP discovery. This strategy, combined with functional analyses, can yield candidate target genes. In support of this, our efforts suggest that investigating the role of Tubb2b in SOX10-positive cells may reveal novel biology within these cell populations.

KEYWORDS:

Enhancer; Neuropathy; Peripheral nerve; SOX10; Schwann cells; TUBB2B; Transcriptional regulation

PMID:
29716548
PMCID:
PMC5930951
DOI:
10.1186/s12864-018-4692-z
[Indexed for MEDLINE]
Free PMC Article

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