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BMC Cancer. 2018 May 2;18(1):495. doi: 10.1186/s12885-018-4410-x.

Staining of E-selectin ligands on paraffin-embedded sections of tumor tissue.

Author information

1
UCIBIO, Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Lisbon, Portugal.
2
CEDOC, Chronic Diseases Research Center, NOVA Medical School/Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Lisbon, Portugal.
3
Hospital CUF Descobertas, Lisbon, Portugal.
4
Centro Hospitalar de Lisboa Central, EPE e Serviço de Anatomia Patológica, Lisbon, Portugal.
5
Departments of Dermatology and Medicine, Brigham & Women's Hospital, Harvard Medical School, Boston, USA.
6
Program of Excellence in Glycosciences, Harvard Medical School, Boston, USA.
7
UCIBIO, Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Lisbon, Portugal. p.videira@fct.unl.pt.
8
CDG & Allies - Professionals and Patient Associations International Network (CDG & Allies - PPAIN), Caparica, Portugal. p.videira@fct.unl.pt.

Abstract

BACKGROUND:

The E-selectin ligands expressed by cancer cells mediate adhesion of circulating cancer cells to endothelial cells, as well as within tissue microenvironments important for tumor progression and metastasis. The identification of E-selectin ligands within cancer tissue could yield new biomarkers for patient stratification and aid in identifying novel therapeutic targets. The determinants of selectin ligands consist of sialylated tetrasaccharides, the sialyl Lewis X and A (sLeX and sLeA), displayed on protein or lipid scaffolds. Standardized procedures for immunohistochemistry make use of the antibodies against sLeX and/or sLeA. However, antibody binding does not define E-selectin binding activity.

METHODS:

In this study, we developed an immunohistochemical staining technique, using E-selectin-human Ig Fc chimera (E-Ig) to characterize the expression and localization of E-selectin binding sites on paraffin-embedded sections of different cancer tissue.

RESULTS:

E-Ig successfully stained cancer cells with high specificity. The E-Ig staining show high reactivity scores in colon and lung adenocarcinoma and moderate reactivity in triple negative breast cancer. Compared with reactivity of antibody against sLeX/A, the E-Ig staining presented higher specificity to cancer tissue with better defined borders and less background.

CONCLUSIONS:

The E-Ig staining technique allows the qualitative and semi-quantitative analysis of E-selectin binding activity on cancer cells. The development of accurate techniques for detection of selectin ligands may contribute to better diagnostic and better understanding of the molecular basis of tumor progression and metastasis.

KEYWORDS:

Cancer; E-selectin ligands; Sialyl-Lewis X; Sialyl-Lewis a

PMID:
29716546
PMCID:
PMC5930952
DOI:
10.1186/s12885-018-4410-x
[Indexed for MEDLINE]
Free PMC Article

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