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Org Biomol Chem. 2018 May 15;16(19):3662-3671. doi: 10.1039/c8ob00552d.

Chemo-enzymatic synthesis of isotopically labeled nicotinamide riboside.

Author information

1
Department of Pharmaceutical Sciences, Albany College of Pharmacy and Health Sciences, 261 Mountain View Drive, Colchester, VT 05446, USA. Yana.Cen@acphs.edu.

Abstract

As a cofactor for numerous reactions, NAD+ is found widely dispersed across many maps of cellular metabolism. This core redox role alone makes the biosynthesis of NAD+ of great interest. Recent studies have revealed new biological roles for NAD+ as a substrate for diverse enzymes that regulate a broad spectrum of key cellular tasks. These NAD+-consuming enzymes further highlight the importance of understanding NAD+ biosynthetic pathways. In this study, we developed a chemo-enzymatic synthesis of isotopically labeled NAD+ precursor, nicotinamide riboside (NR). The synthesis of NR isotopomers allowed us to unambiguously determine that NR is efficiently converted to NAD+ in the cellular environment independent of degradation to nicotinamide, and it is incorporated into NAD+ in its intact form. The versatile synthetic method along with the isotopically labeled NRs will provide powerful tools to further decipher the important yet complicated NAD+ metabolism.

PMID:
29714801
PMCID:
PMC6054311
[Available on 2019-05-15]
DOI:
10.1039/c8ob00552d
[Indexed for MEDLINE]

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