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Curr Stem Cell Res Ther. 2018 May 1. doi: 10.2174/1574888X13666180501120416. [Epub ahead of print]

Epithelial in vitro differentiation of Mesenchymal Stem Cells.

Author information

1
Cell Production and Tissue Engineering Unit. Biosanitary Institute of Granada (ibs.GRANADA). Virgen de las Nieves University Hospital. Granada. Spain.
2
Cell Production and Tissue Engineering Unit. BioCell Production and Tissue Engineering Unit. Biosanitary Institute of Granada (ibs.GRANADA). Virgen de las Nieves University Hospital. Granada. Spain.

Abstract

Mesenchymal stem cells or mesenchymal stromal cells (MSCs) are non-hematopoietic stromal cells that reside in and have been isolated from a variety of adult or fetal tissues such as adipose tissue, bone marrow and umbilical cord Wharton's jelly, among others. Because they are a heterogeneous population, International Society for Cellular Therapy has established 3 minimum criteria to characterize MSCs in vitro: i) adherence to plastic, ii) differentiation potential (osteogenic, chondrogenic and adipogenic lineages) and iii) expression of specific surface antigens (CD73+, CD90+, CD105+, CD34-, CD45-, CD11b-, CD14-, CD19-, CD79a-, HLA-DR-). Because of these characteristics, MSCs are useful for different applications and studies, most of them related with regenerative biomedicine. Epithelial in vitro differentiation of MSCs, for clinical use, is one of the main objectives in this field, due to, on the one hand, the difficulties to stablish epithelial cell cultures and, on the other hand, the immunomodulatory capacity of MSCs that could increase the success of transplantation. According to this and the information compiled from bibliography, production of epithelial cells differentiated in vitro from MSCs is a complex procedure and a lot of techniques and culture media are necessary to explore. The objective of this review is to show the different methods of epithelial in vitro differentiation and remark the need of continue studying for being capable of establish specific cell lines of epithelial cells differentiated from autogenic or allogenic MSCs.

KEYWORDS:

Adult stem cell; Epithelial cells; Epithelial in vitro differentiation; Mesenchymal stem cells; Regenerative biomedicine; Stem cell.

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