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Biomed Pharmacother. 2018 Jun;102:988-995. doi: 10.1016/j.biopha.2018.03.122. Epub 2018 Apr 5.

MicroRNA-361-5p inhibits papillary thyroid carcinoma progression by targeting ROCK1.

Author information

1
Department of Thyroid Surgery, The First Hospital of Jilin University, 71# Xinmin Street, Changchun 130021, China.
2
Departments of Hepatobiliary Surgery, China-Japan Union Hospital of Jilin University, 126 Xiantai Street, Changchun 130033, China. Electronic address: jt214069@263.net.
3
Department of Thyroid Surgery, The First Hospital of Jilin University, 71# Xinmin Street, Changchun 130021, China. Electronic address: chenguang518@aliyun.com.

Abstract

MicroRNAs function as key regulators in various human cancers, including papillary thyroid cancer (PTC). MiR-361-5p has been proved to be a tumor suppressor in multiple cancers. However, the function of miR-361-5p in PTC remains unknown. In this study, we aimed to determine the function of miR-361-5p in PTC progression, and elaborate the mechanism by which miR-361-5p acts in PTC. Here, we report that miR-361-5p expression levels were significantly downregulated in PTC tissues and cell lines, as detected by reverse transcription-quantitative polymerase chain reaction (qRT-PCR) analysis. Functional analysis revealed that overexpression of miR-361-5p significantly inhibited cell proliferation, migration, invasion in vitro, as well as suppressed tumor growth in vivo. Bioinformatic analysis showed that Rho-associated coiled-coil kinase 1 (ROCK1) was a predicted target of miR-361-5p, which was further validated by the dual-luciferase reporter assay, qRT-PCR, and western blot analysis. In addition, an inverse expression pattern was also observed between miR-361-5p and ROCK1 in a cohort of PTC tissues. Rescue experiments showed that restoration of ROCK1 expression significantly reversed the suppressive effect of miR-361-5p on cell proliferation, migration, and invasion in PTC cells. Taken together, these findings suggest that miR-361-5p is a novel potential therapeutic target for thyroid cancer.

KEYWORDS:

Key words; Papillary thyroid carcinoma; ROCK1; Thyroid cancer; miR-361-5p

PMID:
29710554
DOI:
10.1016/j.biopha.2018.03.122
[Indexed for MEDLINE]

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