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Biol Pharm Bull. 2018;41(5):828-832. doi: 10.1248/bpb.b18-00046.

Comparison of Human Selenoprotein P Determinants in Serum between Our Original Methods and Commercially Available Kits.

Author information

1
Systems Life Sciences Laboratory, Department of Medical Life Systems, Faculty of Life and Medical Sciences, Doshisha University.
2
Department of Endocrinology and Metabolism, Kanazawa University Graduate School of Medical Sciences.
3
PRESTO, Japan Science and Technology Agency.
4
Department of Disease Control and Homeostasis, Kanazawa University Graduate School of Medical Sciences.

Abstract

Selenoprotein P (SeP) is a selenium (Se)-rich extracellular protein. SeP is identified as a hepatokine, causing insulin resistance in type 2 diabetes. Thus, the measurement of SeP in serum has received much attention, and several enzyme-linked immunosorbent assay (ELISA) kits for SeP determination are now commercially available. In the present study, we determined the serum SeP levels by our original ELISA and sol particle homogeneous immunoassay (SPIA) methods and also by commercially available kits, and these determinants were compared. We found a kit-dependent correlation of the determinants with our methods. These results suggest that the selection of kit is critical for comparison with our previous reports and for discussing the relationship between the serum SeP levels and disease condition.

KEYWORDS:

commercially available kit; enzyme-linked immunosorbent assay (ELISA); monoclonal antibody; selenoprotein P; sol particle homogeneous immunoassay (SPIA)

PMID:
29709922
DOI:
10.1248/bpb.b18-00046
[Indexed for MEDLINE]
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