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Int J Biol Macromol. 2018 Aug;115:933-939. doi: 10.1016/j.ijbiomac.2018.04.139. Epub 2018 Apr 28.

Bioaccessibility and inhibitory effects on digestive enzymes of carnosic acid in sage and rosemary.

Author information

1
Food Engineering Department, Engineering Faculty, Ege University, 35100 Bornova, Izmir, Turkey. Electronic address: pinarercan@yandex.com.
2
Food Engineering Department, Engineering Faculty, Ege University, 35100 Bornova, Izmir, Turkey. Electronic address: sedef.el@ege.edu.tr.

Abstract

In this study, the aim was to determine the bioaccessibilities of carnosic acid in sage and rosemary and in vitro inhibitory effects of these samples on lipid and starch digestive enzymes by evaluating the lipase, α-amylase and α-glucosidase enzyme inhibition activities. The content of carnosic acid in rosemary (18.72 ± 0.33 mg/g) was found to be higher than that content of that in sage (3.76 ± 0.13 mg/g) (p < 0.05). The carnosic acid bioaccessibilities were found as 45.10 ± 1.88% and 38.32 ± 0.21% in sage and rosemary, respectively. The tested sage and rosemary showed inhibitory activity against α-glucosidase (Concentration of inhibitor required to produce a 50% inhibition of the initial rate of reaction - IC50 88.49 ± 2.35, 76.80 ± 1.68 μg/mL, respectively), α-amylase (IC50 107.65 ± 12.64, 95.65 ± 2.73 μg/mL, respectively) and lipase (IC50 6.20 ± 0.63, 4.31 ± 0.62 μg/mL, respectively). Furthermore, to the best of our knowledge, this is the first work that carnosic acid standard equivalent inhibition capacities (CAEIC50) for these food samples were determined and these values were in agreement with the IC50 values. These results show that sage and rosemary are potent inhibitors of lipase, α-amylase and α-glucosidase digestive enzymes.

KEYWORDS:

4-Nitrophenyl α-d-glucopyranoside (PNPG) (PubChem CID: 92969); Carnosic acid; Carnosic acid (PubChem CID: 65126); Hexane (PubChem CID:8058); In vitro bioaccessibility; Inhibitors; Lipase; Methanol (PubChem CID: 887); Pefabloc® SC (PubChem CID: 186136); Phosphoric acid (PubChem CID:1004); α-Amylase; α-Glucosidase

PMID:
29709538
DOI:
10.1016/j.ijbiomac.2018.04.139
[Indexed for MEDLINE]

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