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PLoS One. 2018 Apr 30;13(4):e0196470. doi: 10.1371/journal.pone.0196470. eCollection 2018.

B cells response directed against Cut4 and CFP21 lipolytic enzymes in active and latent tuberculosis infections.

Author information

1
Pathogenesis and Control of Chronic Infections, INSERM, EFS, Université de Montpellier, CHU Montpellier, Montpellier, France.
2
PhyMedExp, INSERM, EFS, Université de Montpellier, CHU Montpellier, Montpellier, France.
3
Laboratory of Enzymology at Interfaces and Physiology of Lipolysis, CNRS, Université Aix-Marseille, France.
4
Institute of Research on Infection of Montpellier, CNRS, INSERM, Université de Montpellier, Montpellier, France.

Abstract

BACKGROUND:

Better understanding of the immune response directed against Mycobacterium tuberculosis (Mtb) is critical for development of vaccine strategies and diagnosis tests. Previous studies suggested that Mtb enzymes involved in lipid metabolism, are associated with persistence and/or reactivation of dormant bacilli.

METHODS:

Circulating antibodies secreting cells (ASCs), memory B cells, and antibodies directed against Cut4 (Rv3452) and CFP21 (Rv1984c) antigens were explored in subjects with either active- or latent-tuberculosis (LTB), and in Mtb-uninfected individuals.

RESULTS:

Circulating anti-Cut4 ASCs were detected in 11/14 (78.6%) subjects from the active TB group vs. 4/17 (23.5%) from the LTB group (p = 0.001). Anti-CFP21 ASCs were found in 11/14 (78.6%) active TB vs. in 5/17 (29.4%) LTB cases (p = 0.01). Circulating anti-Cut4 and anti-CFP21 ASCs were not detected in 38 Mtb uninfected controls. Memory B cells directed against either Cut4 or CFP21 were identified in 8/11 (72.7%) and in 9/11 (81.8%) subjects with LTB infection, respectively, and in 2/6 Mtb uninfected individuals (33.3%). High level of anti-Cut4 and anti-CFP21 IgG were observed in active TB cases.

CONCLUSION:

Circulating IgG SCs directed against Cut4 or CFP21 were mostly detected in patients presenting an active form of the disease, suggesting that TB reactivation triggers an immune response against these two antigens.

PMID:
29709002
PMCID:
PMC5927435
DOI:
10.1371/journal.pone.0196470
[Indexed for MEDLINE]
Free PMC Article

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