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J Photochem Photobiol B. 2018 Jun;183:127-132. doi: 10.1016/j.jphotobiol.2018.04.003. Epub 2018 Apr 6.

Morphological and genetical changes of endothelial progenitor cells after in-vitro conversion into photoreceptors.

Author information

1
Opthalmology Department, First Affiliated Hospital of Xi'an Jiao Tong University, China.
2
Department of Medical Microbiology and Parasitology, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia. Electronic address: alsaeedyhiba@gmail.com.
3
Department of Medical Microbiology and Parasitology, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.
4
Department of Chemical and Materials Engineering, National Central University, Jhong-li, Taoyuan 32001, Taiwan; Department of Botany and Microbiology, King Saud University, Riyadh 11451, Saudi Arabia.
5
Department of Botany and Microbiology, King Saud University, Riyadh 11451, Saudi Arabia.
6
Department of Clinical Laboratory Sciences, College of Applied Medical Science, Aljouf University, Sakaka, P.O. Box 2014, Aljouf Province, Saudi Arabia.
7
Department of Biomedical Science, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia; Genetics and Regenerative Medicine Research Center, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia (UPM), 43400 Serdang, Selangor, Malaysia.
8
Opthalmology Department, First Affiliated Hospital of Xi'an Jiao Tong University, China. Electronic address: peich71@163.com.

Abstract

BACKGROUND:

Retinal degeneration is a condition ensued by various ocular disorders such as artery occlusion, diabetic retinopathy, retrolental fibroplasia and retinitis pigmentosa which cause abnormal loss of photoreceptor cells and lead to eventual vision impairment. No efficient treatment has yet been found, however, the use of stem cell therapy such as bone marrow and embryonic stem cells has opened a new treatment modality for retinal degenerative diseases. The major goal of this study is to analyze the potential of endothelial progenitor cells derived from bone marrow to differentiate into retinal neural cells for regenerative medicine purposes.

METHODS:

In this study, endothelial progenitor cells were induced in-vitro with photoreceptor growth factor (taurine) for 21 days. Subsequently, the morphology and gene expression of CRX and RHO of the photoreceptors-induced EPCs were examined through immunostaining assay.

FINDINGS:

The results indicated that the induced endothelial progenitor cells demonstrated positive gene expression of CRX and RHO. Our findings suggested that EPC cells may have a high advantage in cell replacement therapy for treating eye disease, in addition to other neural diseases, and may be a suitable cell source in regenerative medicine for eye disorders.

KEYWORDS:

Endothelial progenitor cells; Immunocytochemistry; Photoreceptors; Retinal degeneration

[Indexed for MEDLINE]

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