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ACS Appl Mater Interfaces. 2018 May 16;10(19):16233-16237. doi: 10.1021/acsami.7b18855. Epub 2018 May 3.

Electronic Cortisol Detection Using an Antibody-Embedded Polymer Coupled to a Field-Effect Transistor.

Author information

1
Department of Materials Science and Engineering , Johns Hopkins University , 3400 North Charles Street , Baltimore , Maryland 21218-2608 , United States.

Abstract

A field-effect transistor-based cortisol sensor was demonstrated in physiological conditions. An antibody-embedded polymer on the remote gate was proposed to overcome the Debye length issue (λD). The sensing membrane was made by linking poly(styrene- co-methacrylic acid) (PSMA) with anticortisol before coating the modified polymer on the remote gate. The embedded receptor in the polymer showed sensitivity from 10 fg/mL to 10 ng/mL for cortisol and a limit of detection (LOD) of 1 pg/mL in 1× PBS where λD is 0.2 nm. A LOD of 1 ng/mL was shown in lightly buffered artificial sweat. Finally, a sandwich ELISA confirmed the antibody binding activity of antibody-embedded PSMA.

KEYWORDS:

Debye screening length; antibody-embedded polymer; cortisol; perspiration; poly(styrene-co-methacrylic acid); transistor biosensor

PMID:
29701946
PMCID:
PMC6026499
DOI:
10.1021/acsami.7b18855
[Indexed for MEDLINE]
Free PMC Article

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