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Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2018 Mar 28;43(3):240-245. doi: 10.11817/j.issn.1672-7347.2018.03.002.

[Expression profiles of CaMKIIγ during 
osteoclast differentiation].

[Article in Chinese; Abstract available in Chinese from the publisher]

Author information

1
Department of Oral and Maxillofacial Surgery, College of Stomatology, North China University of Science and Technology, Tangshan Hebei 063000, China.
2
Department of Pathology, College of Basic Medicine, North China University of Science and Technology, Tangshan Hebei 063000, China.

Abstract

in English, Chinese

To study the expression profiles and the role of Ca2+/calmodulin-dependent protein kinase IIγ (CaMKIIγ) during osteoclast differentiation.
 Methods: Mouse RAW264.7 cells were induced for osteoclastogenesis with 50 ng/mL receptor activator of nuclear factor-κB ligand (RANKL) and the cells were harvested at 0, 1, 3 and 5 days after induction. Tartrate-resistant acid phosphotase staining was performed to verify osteoclasts formation. RT-PCR, Western blot and immunofluorescent cytochemistry were used to detect the CaMKIIγ gene expression during osteoclastogenesis.
 Results: The osteoclasts were formed at day 3 under RANKL induction and more osteoclasts were observed at day 5. At day 0, 1, 3 and 5, the relative level of CaMKIIγ mRNA were (1.067±0.179), (1.840±0.070), (9.493±0.453) and (30.767±0.573), respectively, and the relative protein level were (0.454±0.065), (0.613±0.021), (0.858±0.019) and (0.980±0.023), respectively. CaMKIIγ expression was increased in a time-dependent manner except relative protein level at day 1 (P<0.01), which showed no significant difference at day 0 (P>0.05). Immunofluorescence assay showed that CaMKIIγ protein was also increased with differentiation of osteoclasts.
 Conclusion: The CaMKIIγ expression was increased in a time-depended manner during osteoclast differentiation and it might play a vital role during osteoclastogenesis.

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