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BMC Vet Res. 2018 Apr 24;14(1):138. doi: 10.1186/s12917-018-1457-5.

Tween-20 transiently changes the surface morphology of PK-15 cells and improves PCV2 infection.

Hua T1,2,3,4, Zhang X1,2,3,4, Tang B1,2,3,4, Chang C1,2,3,4, Liu G1,2,3,4, Feng L1,2,3,4, Yu Y1,2,3,4, Zhang D5,6,7,8, Hou J9,10,11,12.

Author information

1
Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China.
2
National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China.
3
Key lab of Food Quality and Safety of Jiangsu Province-State Key laboratory Breeding Base, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China.
4
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China.
5
Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China. 782644144@qq.com.
6
National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China. 782644144@qq.com.
7
Key lab of Food Quality and Safety of Jiangsu Province-State Key laboratory Breeding Base, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China. 782644144@qq.com.
8
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China. 782644144@qq.com.
9
Institute of Veterinary Immunology & Engineering, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, China. houjiboccvv@163.com.
10
National Research Center of Engineering and Technology for Veterinary Biologicals, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China. houjiboccvv@163.com.
11
Key lab of Food Quality and Safety of Jiangsu Province-State Key laboratory Breeding Base, Jiangsu Academy of Agricultural Science, Nanjing, 210014, China. houjiboccvv@163.com.
12
Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China. houjiboccvv@163.com.

Abstract

BACKGROUND:

Low concentrations of nonionic surfactants can change the physical properties of cell membranes, and thus and in turn increase drug permeability. Porcine circovirus 2 (PCV2) is an extremely slow-growing virus, and PCV2 infection of PK-15 cells yields very low viral titers. The present study investigates the effect of various nonionic surfactants, namely, Tween-20, Tween-28, Tween-40, Tween-80, Brij-30, Brij-35, NP-40, and Triton X-100 on PCV2 infection and yield in PK-15 cells.

RESULT:

Significantly increased PCV2 infection was observed in cells treated with Tween-20 compared to those treated with Tween-28, Tween-40, Brij-30, Brij-35, NP-40, and Triton X-100 (p < 0.01). Furthermore, 24 h incubation with 0.03% Tween-20 has shown to induce significant cellular morphologic changes (cell membrane underwent slight intumescence and bulged into a balloon, and the number of microvilli decreased), as well as to increase caspase-3 activity and to decrease cell viability in PCV2-infected PK-15 cells cmpared to control group; all these changes were restored to normal after Tween-20 has been washed out from the plate.

CONCLUSION:

Our data demonstrate that Tween-20 transiently changes the surface morphology of PK-15 cells and improves PCV2 infection. The findings of the present study may be utilized in the development of a PCV2 vaccine.

KEYWORDS:

Cellular morphologic change; Nonionic surfactant; PCV2; Tween-20; Viral infection

PMID:
29699558
PMCID:
PMC5921416
DOI:
10.1186/s12917-018-1457-5
[Indexed for MEDLINE]
Free PMC Article

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