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Biochem Biophys Res Commun. 2018 Jun 18;501(1):85-91. doi: 10.1016/j.bbrc.2018.04.171. Epub 2018 May 4.

Glycine confers neuroprotection through PTEN/AKT signal pathway in experimental intracerebral hemorrhage.

Author information

1
Department of Physiology, Collaborative Innovation Center for Brain Science, School of Basic Medical Sciences, Wuhan University School of Medicine, 185 Donghu Street, Wuhan 430071, China; Department of Biomedical Engineering, School of Basic Medical Sciences, WuhanUniversity, Wuhan 430071, China; Department of Physiology, School of Basic Medical Sciences, Hubei University of Medicine, 30 South Renmin Road, Shiyan, Hubei, 442000 China.
2
Department of Physiology, Collaborative Innovation Center for Brain Science, School of Basic Medical Sciences, Wuhan University School of Medicine, 185 Donghu Street, Wuhan 430071, China; Department of Neurology, The Central Hospital of Wuhan, Tongji Medical College of Huazhong University of Science & Technology, 26 Shengli Street, Wuhan, 430013, China.
3
Department of Physiology, Collaborative Innovation Center for Brain Science, School of Basic Medical Sciences, Wuhan University School of Medicine, 185 Donghu Street, Wuhan 430071, China.
4
Department of Physiology, Collaborative Innovation Center for Brain Science, School of Basic Medical Sciences, Wuhan University School of Medicine, 185 Donghu Street, Wuhan 430071, China; Department of Physiology, School of Basic Medical Sciences, Hubei University of Medicine, 30 South Renmin Road, Shiyan, Hubei, 442000 China.
5
Institute of Neuroregeneration& Neurorehabilitation, Department of Neurosurgery of the Affiliated Hospital, Qingdao University, 308 Ningxia Street, Qingdao, 266071, China.
6
Department of Biomedical Engineering, School of Basic Medical Sciences, WuhanUniversity, Wuhan 430071, China. Electronic address: yunchen@whu.edu.cn.
7
Institute of Neuroregeneration& Neurorehabilitation, Department of Neurosurgery of the Affiliated Hospital, Qingdao University, 308 Ningxia Street, Qingdao, 266071, China. Electronic address: qwanwh@hotmail.com.

Abstract

Glycine has been shown to protect against ischemic stroke through various mechanisms. Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) which antagonize Akt-dependent cell survival has been linked to neuronal damage. However, whether glycine has a neuroprotective property in intracerebral hemorrhage (ICH) was unknown. This study aimed to determine the protective effect of glycine in rats ICH. Adult male Sprague-Dawley (SD) rats were subjected to left striatum infusion of autologous blood. ICH animals received glycine (0.2-3 mg/kg, icv) at 1 h after ICH with or without pre-injection of Akt Inhibitor IV (100 μM, 2 μl, icv) 0.5 h prior to glycine treatment. Our results showed that in the perihematomal area PTEN was up-regulated in the early stage after ICH. However, glycine treatment decreased PTEN protein level and increased the phosphorylation level of AKT (p-AKT) in the perihematomal area. With the administration of glycine, neuronal death was significantly reduced and Evans blue leakage was alleviated as well as the brain edema after ICH. Moreover, hematoma volume was decreased and neurobehavioral outcome was improved. Nevertheless, Akt Inhibitor IV abolished the neuroprotective effects of glycine after ICH. Together, our findings demonstrate, for the first time, the protective role of glycine on ICH rats, and suggest that the neuroprotective effect of glycine was mediated through PTEN/Akt signal pathway.

KEYWORDS:

Glycine; Intracerebral hemorrhage; Neuroprotection; Phosphatase and tensin homolog deleted on chromosome 10

PMID:
29698679
DOI:
10.1016/j.bbrc.2018.04.171
[Indexed for MEDLINE]

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