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Biochem Biophys Res Commun. 2018 Jun 12;500(4):852-859. doi: 10.1016/j.bbrc.2018.04.173. Epub 2018 May 1.

Long noncoding RNA HOTTIP cooperates with CCCTC-binding factor to coordinate HOXA gene expression.

Author information

1
Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 South Jingming Road, Chenggong District, Kunming, Yunnan Province, 650500, China.
2
Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 South Jingming Road, Chenggong District, Kunming, Yunnan Province, 650500, China; Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, No. 1 Boda Road, Yuhua Area, Chenggong District, Kunming, Yunnan, 650500, China.
3
Yunnan Key Laboratory of Primate Biomedical Research, Institute of Primate Translational Medicine, Kunming University of Science and Technology, No. 1 Boda Road, Yuhua Area, Chenggong District, Kunming, Yunnan, 650500, China.
4
College of Biological Big Data, Yunan Agricultural University, 452 Fengyuan Road, Kunming, Yunnan Province, 650201, China.
5
Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 South Jingming Road, Chenggong District, Kunming, Yunnan Province, 650500, China. Electronic address: lianbing.lin36@yahoo.com.

Abstract

The spatiotemporal control of HOX gene expression is dependent on positional identity and often correlated to their genomic location within each loci. Maintenance of HOX expression patterns is under complex transcriptional and epigenetic regulation, which is not well understood. Here we demonstrate that HOTTIP, a lincRNA transcribed from the 5' edge of the HOXA locus, physically associates with the CCCTC-binding factor (CTCF) that serves as an insulator by organizing HOXA cluster into disjoint domains, to cooperatively maintain the chromatin modifications of HOXA genes and thus coordinate the transcriptional activation of distal HOXA genes in human foreskin fibroblasts. Our results reveal the functional connection of HOTTIP and CTCF, and shed light on lincRNAs in gene activation and CTCF mediated chromatin organization.

KEYWORDS:

Chromatin boundary; HOX genes; Positional identity; Transcriptional regulation; ncRNAs

PMID:
29698677
DOI:
10.1016/j.bbrc.2018.04.173
[Indexed for MEDLINE]

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