Distribution Analysis of Anthocyanins, Sugars, and Organic Acids in Strawberry Fruits Using Matrix-Assisted Laser Desorption/Ionization-Imaging Mass Spectrometry

J Agric Food Chem. 2018 May 16;66(19):4958-4965. doi: 10.1021/acs.jafc.8b00853. Epub 2018 May 2.

Abstract

Anthocyanins, sugars, and organic acids contribute to the appearance, health benefits, and taste of strawberries. However, their spatial distribution in the ripe fruit has been fully unrevealed. Therefore, we performed matrix-assisted laser desorption/ionization, MALDI-IMS, analysis to investigate their spatial distribution in ripe strawberries. The detection sensitivity was improved by using the TM-Sprayer for matrix application. In the receptacle, pelargonidins were distributed in the skin, cortical, and pith tissues, whereas cyanidins and delphinidins were slightly localized in the skin. In the achene, mainly cyanidins were localized in the outside of the skin. Citric acid was mainly distributed in the upper and bottom side of cortical tissue. Although hexose was distributed almost equally throughout the fruits, sucrose was mainly distributed in the upper side of cortical and pith tissues. These results suggest that using the TM-Sprayer in MALDI-IMS was useful for microscopic distribution analysis of anthocyanins, sugars, and organic acids in strawberries.

Keywords: Strawberry fruits; anthocyanins; imaging mass spectrometry; matrix-assisted laser desorption/ionization; organic acids; sugars.

Publication types

  • Evaluation Study

MeSH terms

  • Acids / analysis*
  • Anthocyanins / analysis*
  • Fragaria / chemistry*
  • Fruit / chemistry
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Sugars / analysis*

Substances

  • Acids
  • Anthocyanins
  • Sugars