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Front Cell Dev Biol. 2018 Apr 11;6:39. doi: 10.3389/fcell.2018.00039. eCollection 2018.

Isolation and Characterization of Neural Crest-Derived Stem Cells From Adult Ovine Palatal Tissue.

Author information

1
Stem Cell Biology and Regenerative Medicine, School of Pharmacy, University of Reading, Reading, United Kingdom.
2
Stem Cell Lab, Department for Personalized Medicine, Scientific Innovation Centre, Stavropol State Medical University, Stavropol, Russia.
3
Centre for Dairy Research, School of Agriculture, Policy and Development, University of Reading, Reading, United Kingdom.
4
Animal, Dairy and Food Chain Sciences Research Group, Centre for Dairy Research, School of Agriculture, Policy and Development, University of Reading, Reading, United Kingdom.
5
Skeletal Muscle Development Group, School of Biological Sciences, University of Reading, Reading, United Kingdom.
6
Periodontology, Department of Dental Medicine, Faculty of Health, University of Witten/Herdecke, Witten, Germany.

Abstract

Adult mammalian craniofacial tissues contain limited numbers of post-migratory neural crest-derived stem cells. Similar to their embryonic counterparts, these adult multipotent stem cells can undergo multi-lineage differentiation and are capable of contributing to regeneration of mesodermal and ectodermal cells and tissues in vivo. In the present study, we describe for the first time the presence of Nestin-positive neural crest-derived stem cells (NCSCs) within the ovine hard palate. We show that these cells can be isolated from the palatal tissue and are able to form neurospheres. Ovine NCSCs express the typical neural crest markers Slug and Twist, exhibit high proliferative and migratory activity and are able to differentiate into α smooth muscle cells and β-III-tubulin expressing ectodermal cells. Finally, we demonstrate that oNCSCs are capable of differentiating into osteogenic, adipogenic and chondrogenic cells. Taken together, our results suggest that oNCSCs could be used as model cells to assess the efficacy and safety of autologous NCSC transplantation in a large animal model.

KEYWORDS:

large animal model; multipotency; neural crest; neural crest-derived stem cells; ovine stem cells

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