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J Clin Microbiol. 2018 Jun 25;56(7). pii: e00053-18. doi: 10.1128/JCM.00053-18. Print 2018 Jul.

Whole-Genome Sequencing of Aggregatibacter Species Isolated from Human Clinical Specimens and Description of Aggregatibacter kilianii sp. nov.

Author information

1
Department of Clinical Microbiology, Aarhus University Hospital, Aarhus, Denmark.
2
Department of Clinical Microbiology, Aalborg University Hospital, Aalborg, Denmark.
3
Institute of Medical Microbiology, University of Zurich, Zurich, Switzerland.
4
Bioinformatics Research Centre, Aarhus University, Aarhus, Denmark.
5
Department of Clinical Microbiology, Aarhus University Hospital, Aarhus, Denmark nielnoer@rm.dk.

Abstract

Aggregatibacter species are commensal bacteria of human mucosal surfaces that are sometimes involved in serious invasive infections. During the investigation of strains cultured from various clinical specimens, we encountered a coherent group of 10 isolates that could not be allocated to any validly named species by phenotype, mass spectrometry, or partial 16S rRNA gene sequencing. Whole-genome sequencing revealed a phylogenetic cluster related to but separate from Aggregatibacter aphrophilus The mean in silico DNA hybridization value for strains of the new cluster versus A. aphrophilus was 56% (range, 53.7 to 58.0%), whereas the average nucleotide identity was 94.4% (range, 93.9 to 94.8%). The new cluster exhibited aggregative properties typical of the genus Aggregatibacter Key phenotypic tests for discrimination of the new cluster from validly named Aggregatibacter species are alanine-phenylalanine-proline arylamidase, N-acetylglucosamine, and β-galactosidase. The name Aggregatibacter kilianii is proposed, with PN_528 (CCUG 70536T or DSM 105094T) as the type strain.

KEYWORDS:

HACEK; Haemophilus; abscesses; average nucleotide identity; phylogeny

PMID:
29695522
PMCID:
PMC6018338
DOI:
10.1128/JCM.00053-18
[Indexed for MEDLINE]
Free PMC Article

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