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J Virol. 2018 Jun 29;92(14). pii: e00333-18. doi: 10.1128/JVI.00333-18. Print 2018 Jul 15.

Critical Role of the Human T-Cell Leukemia Virus Type 1 Capsid N-Terminal Domain for Gag-Gag Interactions and Virus Particle Assembly.

Author information

1
Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA.
2
Pharmacology Graduate Program, University of Minnesota, Minneapolis, Minnesota, USA.
3
Division of Basic Sciences, School of Dentistry, University of Minnesota, Minneapolis, Minnesota, USA.
4
Department of Chemistry & Biochemistry, University of Delaware, Newark, Delaware, USA.
5
School of Physics & Astronomy, University of Minnesota, Minneapolis, Minnesota, USA.
6
Characterization Facility, University of Minnesota, Minneapolis, Minnesota, USA.
7
Institute for Molecular Virology, University of Minnesota, Minneapolis, Minnesota, USA mansky@umn.edu.
8
Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA.

Abstract

The retroviral Gag protein is the main structural protein responsible for virus particle assembly and release. Like human immunodeficiency virus type 1 (HIV-1) Gag, human T-cell leukemia virus type 1 (HTLV-1) has a structurally conserved capsid (CA) domain, including a β-hairpin turn and a centralized coiled-coil-like structure of six α helices in the CA amino-terminal domain (NTD), as well as four α-helices in the CA carboxy-terminal domain (CTD). CA drives Gag oligomerization, which is critical for both immature Gag lattice formation and particle production. The HIV-1 CA CTD has previously been shown to be a primary determinant for CA-CA interactions, and while both the HTLV-1 CA NTD and CTD have been implicated in Gag-Gag interactions, our recent observations have implicated the HTLV-1 CA NTD as encoding key determinants that dictate particle morphology. Here, we have conducted alanine-scanning mutagenesis in the HTLV-1 CA NTD nucleotide-encoding sequences spanning the loop regions and amino acids at the beginning and ends of α-helices due to their structural dissimilarity from the HIV-1 CA NTD structure. We analyzed both Gag subcellular distribution and efficiency of particle production for these mutants. We discovered several important residues (i.e., M17, Q47/F48, and Y61). Modeling implicated that these residues reside at the dimer interface (i.e., M17 and Y61) or at the trimer interface (i.e., Q47/F48). Taken together, these observations highlight the critical role of the HTLV-1 CA NTD in Gag-Gag interactions and particle assembly, which is, to the best of our knowledge, in contrast to HIV-1 and other retroviruses.IMPORTANCE Retrovirus particle assembly and release from infected cells is driven by the Gag structural protein. Gag-Gag interactions, which form an oligomeric lattice structure at a particle budding site, are essential to the biogenesis of an infectious virus particle. The CA domain of Gag is generally thought to possess the key determinants for Gag-Gag interactions, and the present study has discovered several critical amino acid residues in the CA domain of HTLV-1 Gag, an important cancer-causing human retrovirus, which are distinct from that of HIV-1 as well as other retroviruses studied to date. Altogether, our results provide important new insights into a poorly understood aspect of HTLV-1 replication that significantly enhances our understanding of the molecular nature of Gag-Gag interaction determinants crucial for virus particle assembly.

KEYWORDS:

Gag; deltaretrovirus; lentiviruses; morphology; oligomerization; retrovirus; virus assembly

PMID:
29695435
PMCID:
PMC6026748
DOI:
10.1128/JVI.00333-18
[Indexed for MEDLINE]
Free PMC Article

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