Format

Send to

Choose Destination
J Biomed Mater Res A. 2018 Sep;106(9):2481-2493. doi: 10.1002/jbm.a.36444.

Comparative proteomic analyses of human adipose extracellular matrices decellularized using alternative procedures.

Author information

1
Biomedical Science Program, Tulane University School of Medicine, New Orleans, Louisiana.
2
Center for Stem Cell Research & Regenerative Medicine, Tulane University School of Medicine, New Orleans, Louisiana.
3
National Engineering Laboratory for Oral Regenerative Medicine, West China School of Stomatology, Sichuan University, Chengdu, China.
4
Federal University of Sao Paulo, Sao Paulo, SP, Brazil.
5
Department of Biological and Agricultural Engineering, Louisiana State University, Baton Rouge, Louisiana.
6
Department of Chemistry, Xavier University of Louisiana, New Orleans, Louisiana.
7
LaCell LLC, New Orleans, Louisiana.
8
Department of Structural and Cell Biology, , Tulane University School of Medicine, New Orleans, Louisiana.
9
Department of Pharmacology, Tulane University School of Medicine, New Orleans, Louisiana.
10
Department of Chemical and Biochemical Engineering, Western University, London, Ontario, Canada.
11
Department of Anatomy and Cell Biology, Western University, London, Ontario, Canada.
12
Department of Biomedical Engineering, Pennsylvania State University, State College, Pennsylvania.
13
Department of Medicine, Tulane University School of Medicine, New Orleans, Louisiana.
14
Department of Surgery, Tulane University School of Medicine, New Orleans, Louisiana.

Abstract

Decellularized human adipose tissue has potential clinical utility as a processed biological scaffold for soft tissue cosmesis, grafting, and reconstruction. Adipose tissue decellularization has been accomplished using enzymatic-, detergent-, and/or solvent-based methods. To examine the hypothesis that distinct decellularization processes may yield scaffolds with differing compositions, the current study employed mass spectrometry to compare the proteomes of human adipose-derived matrices generated through three independent methods combining enzymatic-, detergent-, and/or solvent-based steps. In addition to protein content, bioscaffolds were evaluated for deoxyribose nucleic acid depletion, extracellular matrix composition, and physical structure using optical density, histochemical staining, and scanning electron microscopy. Mass spectrometry based proteomic analyses identified 25 proteins (having at least two peptide sequences detected) in the scaffolds generated with an enzymatic approach, 143 with the detergent approach, and 102 with the solvent approach, as compared to 155 detected in unprocessed native human fat. Immunohistochemical detection confirmed the presence of the structural proteins actin, collagen type VI, fibrillin, laminin, and vimentin. Subsequent in vivo analysis of the predominantly enzymatic- and detergent-based decellularized scaffolds following subcutaneous implantation in GFP+ transgenic mice demonstrated that the matrices generated with both approaches supported the ingrowth of host-derived adipocyte progenitors and vasculature in a time dependent manner. Together, these results determine that decellularization methods influence the protein composition of adipose tissue-derived bioscaffolds.

KEYWORDS:

adipose tissue; bioscaffold; decellularization; extracellular matrix; mass spectrometry proteomics; regenerative medicine

PMID:
29693792
PMCID:
PMC6158104
[Available on 2019-09-01]
DOI:
10.1002/jbm.a.36444

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center