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Carbohydr Polym. 2018 Jul 15;192:273-281. doi: 10.1016/j.carbpol.2018.03.013. Epub 2018 Mar 9.

Distinct wall polymer deconstruction for high biomass digestibility under chemical pretreatment in Miscanthus and rice.

Author information

1
Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China. Electronic address: 707917973@qq.com.
2
Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China. Electronic address: 479784828@qq.com.
3
Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China. Electronic address: 752624417@qq.com.
4
Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China. Electronic address: chenpeng@mail.hzau.edu.cn.
5
Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China. Electronic address: jenny_8729@163.com.
6
Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China. Electronic address: wym_quiet@yeah.net.
7
Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China. Electronic address: 124364692@qq.com.
8
Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China. Electronic address: yuant@mail.hzau.edu.cn.
9
Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China. Electronic address: lpeng@mail.hzau.edu.cn.
10
Biomass and Bioenergy Research Centre, Huazhong Agricultural University, Wuhan 430070, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China. Electronic address: wyt@mail.hzau.edu.cn.

Abstract

Miscanthus is a leading bioenergy crop and rice provides enormous biomass for biofuels. Using Calcofluor White staining, this work in situ observed an initial lignocellulose hydrolysis in two distinct Miscanthus accessions, rice cultivar (NPB), and Osfc16 mutant after mild chemical pretreatments. In comparison, the M. sin and Osfc16 respectively exhibited weak Calcofluor fluorescence compared to the M. sac and NPB during enzymatic hydrolysis, consistent with the high biomass saccharification detected in vitro. Using xyloglucan-directed monoclonal antibodies (mAbs), xyloglucan deconstruction was observed from initial cellulose hydrolysis, whereas the M. sin and Osfc16 exhibited relatively strong immunolabeling using xylan-directed mAb, confirming previous findings of xylan positive impacts on biomass saccharification. Furthermore, the M. sin showed quick disappearance of RG-I immunolabeling with varied HG labelings between acid and alkali pretreatments. Hence, this study demonstrated a quick approach to explore wall polymer distinct deconstruction for enhanced biomass saccharification under chemical pretreatment in bioenergy crops.

KEYWORDS:

Biomass saccharification; Chemical pretreatment; Glycan-directed antibodies; Immunolabeling; Lignocellulose hydrolysis; Wall polymers

PMID:
29691021
DOI:
10.1016/j.carbpol.2018.03.013
[Indexed for MEDLINE]

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