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Int J Food Microbiol. 2018 Jul 20;277:74-82. doi: 10.1016/j.ijfoodmicro.2018.04.017. Epub 2018 Apr 12.

Impact of modified diamond-like carbon coatings on the spatial organization and disinfection of mixed-biofilms composed of Escherichia coli and Pantoea agglomerans industrial isolates.

Author information

1
LEPABE - Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal.
2
Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France.
3
LEPABE - Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal. Electronic address: filipem@fe.up.pt.

Abstract

This work investigated the effects of diamond-like carbon (DLC) coatings on the architecture and biocide reactivity of dual-species biofilms mimicking food processing contaminants. Biofilms were grown using industrial isolates of Escherichia coli and Pantoea agglomerans on bare stainless steel (SST) and on two DLC surface coatings (a-C:H:Si:O designated by SICON® and a-C:H:Si designated by SICAN) in order to evaluate their antifouling activities. Quantification and spatial organization in single- and dual-species biofilms were examined by confocal laser scanning microscopy (CLSM) using a strain specific labelling procedure. Those assays revealed that the E. coli isolate exhibited a higher adhesion to the modified surfaces and a decreased susceptibility to disinfectant in presence of P. agglomerans than alone in axenic culture. While SICON® reduced the short-term growth of E. coli in axenic conditions, both DLC surfaces increased the E. coli colonization in presence of P. agglomerans. However, both modified surfaces triggered a significantly higher log reduction of E. coli cells within mixed-species biofilms, thus the use of SICON® and SICAN surfaces may be a good approach to facilitate the disinfection process in critical areas of food processing plants. This study presents a new illustration of the importance of interspecies interactions in surface-associated community functions, and of the need to evaluate the effectiveness of hygienic strategies with relevant multi-species consortia.

KEYWORDS:

Confocal laser scanning microscopy; Diamond-like carbon coatings; Disinfection; Escherichia coli; Multi-species biofilm; Pantoea agglomerans

[Indexed for MEDLINE]

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