Format

Send to

Choose Destination
Dev Cell. 2018 Apr 23;45(2):170-182.e7. doi: 10.1016/j.devcel.2018.03.022.

Mechanosensation Dynamically Coordinates Polar Growth and Cell Wall Assembly to Promote Cell Survival.

Author information

1
Institut Jacques Monod, CNRS UMR7592 and Université Paris Diderot, 15 rue Hélène Brion, 75205 Paris, France.
2
Laboratoire Matière et Systèmes Complexes, UMR7057, CNRS and Université Paris Diderot, 75205 Paris, France.
3
Reproduction et Développement des Plantes, Université de Lyon, ENS de Lyon, UCB Lyon I, INRA, CNRS, 46 Allée d'Italie, 69364 Lyon, France. Electronic address: arezki.boudaoud@ens-lyon.fr.
4
Institut Jacques Monod, CNRS UMR7592 and Université Paris Diderot, 15 rue Hélène Brion, 75205 Paris, France. Electronic address: nicolas.minc@ijm.fr.

Abstract

How growing cells cope with size expansion while ensuring mechanical integrity is not known. In walled cells, such as those of microbes and plants, growth and viability are both supported by a thin and rigid encasing cell wall (CW). We deciphered the dynamic mechanisms controlling wall surface assembly during cell growth, using a sub-resolution microscopy approach to monitor CW thickness in live rod-shaped fission yeast cells. We found that polar cell growth yielded wall thinning and that thickness negatively influenced growth. Thickness at growing tips exhibited a fluctuating behavior with thickening phases followed by thinning phases, indicative of a delayed feedback promoting thickness homeostasis. This feedback was mediated by mechanosensing through the CW integrity pathway, which probes strain in the wall to adjust synthase localization and activity to surface growth. Mutants defective in thickness homeostasis lysed by rupturing the wall, demonstrating its pivotal role for walled cell survival.

KEYWORDS:

cell growth; cell mechanics; cell wall; fission yeast; growth; polarity; super-resolution imaging

PMID:
29689193
DOI:
10.1016/j.devcel.2018.03.022
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center