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Methods Mol Biol. 2018;1773:167-171. doi: 10.1007/978-1-4939-7799-4_14.

Isolation of Murine Adipose-Derived Stromal/Stem Cells Using an Explant Culture Method.

Li J1,2,3, Li H3, Tian W4.

Author information

1
Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, LA, USA.
2
LaCell LLC, New Orleans, LA, USA.
3
National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
4
National Engineering Laboratory for Oral Regenerative Medicine, West China Hospital of Stomatology, Sichuan University, Chengdu, China. drtwd@sina.com.

Abstract

Adipose tissue provides valuable cell source for tissue engineering, regenerative medicine, and adipose tissue biology studies. The most widely used adipose-derived stromal/stem cells (ASCs) isolation protocol involves enzymatic digestion with collagenase. However, the yield of the method is poor even impossible to collect enough stromal vascular fraction (SVF) for expansion when the sample size is small, for instance only new born mice is available for cell culture. Here, we describe an efficient protocol for the isolation and expansion of ASCs using explant culture.

KEYWORDS:

Adipose-derived stromal/stem cells (ASCs); Collagenase; Explant; Mesenchymal stem cells (MSCs); Murine

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