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Methods Mol Biol. 2018;1773:31-39. doi: 10.1007/978-1-4939-7799-4_4.

Differentiation of Brown Adipocyte Progenitors Derived from Human Induced Pluripotent Stem Cells.

Author information

1
Faculté de Médecine, Université Nice Sophia Antipolis, iBV, UMR CNRS/INSERM, Nice Cedex 2, France.
2
Department of Biology, Faculty of Sciences II, Lebanese University, Fanar, Lebanon.
3
Faculté de Médecine, Université Nice Sophia Antipolis, iBV, UMR CNRS/INSERM, Nice Cedex 2, France. dani@unice.fr.

Abstract

In this chapter, we describe a protocol to induce at a high rate the differentiation of brown/brown like adipocyte progenitors (BAPs) derived from human induced pluripotent stem cells (hiPSCs). We also describe culture conditions to maintain hiPSCs and to derive hiPSC-BAPs.This novel culture system provides an unlimited source of human brown adipocytes and a unique means for studying events regulating the generation and recruitment of human BAPs.

KEYWORDS:

Adipocyte; Adipocyte progenitors; Brown adipocytes; Differentiation; Human induced pluripotent stem cells

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