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3 Biotech. 2018 May;8(5):220. doi: 10.1007/s13205-018-1245-8. Epub 2018 Apr 16.

Development of cleaved amplified polymorphic sequence (CAPS) and high-resolution melting (HRM) markers from the chloroplast genome of Glycyrrhiza species.

Author information

1
1Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science (NIHHS), Rural Development Administration (RDA), Eumseong, 27709 Republic of Korea.
2
2Soil and Fertilizer Division, National Institute of Agricultural Science (NIAS), Rural Development Administration (RDA), Wanju, 55365 Republic of Korea.
3
3National Agrobiodiversity Center, National Institute of Agricultural Sciences (NIAS), Rural Development Administration (RDA), Jeonju, 54874 Republic of Korea.
4
4Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju, 28644 Republic of Korea.
#
Contributed equally

Abstract

Licorice (Glycyrrhiza glabra) is an important medicinal crop often used as health foods or medicine worldwide. The molecular genetics of licorice is under scarce owing to lack of molecular markers. Here, we have developed cleaved amplified polymorphic sequence (CAPS) and high-resolution melting (HRM) markers based on single nucleotide polymorphisms (SNP) by comparing the chloroplast genomes of two Glycyrrhiza species (G. glabra and G. lepidota). The CAPS and HRM markers were tested for diversity analysis with 24 Glycyrrhiza accessions. The restriction profiles generated with CAPS markers classified the accessions (2-4 genotypes) and melting curves (2-3) were obtained from the HRM markers. The number of alleles and major allele frequency were 2-6 and 0.31-0.92, respectively. The genetic distance and polymorphism information content values were 0.16-0.76 and 0.15-0.72, respectively. The phylogenetic relationships among the 24 accessions were estimated using a dendrogram, which classified them into four clades. Except clade III, the remaining three clades included the same species, confirming interspecies genetic correlation. These 18 CAPS and HRM markers might be helpful for genetic diversity assessment and rapid identification of licorice species.

KEYWORDS:

CAPS; Genetic diversity; HRM; Licorice; SNP

PMID:
29682439
PMCID:
PMC5902340
[Available on 2019-05-01]
DOI:
10.1007/s13205-018-1245-8

Conflict of interest statement

Compliance with ethical standardsThe authors declare that they have no conflict of interest.

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