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Stem Cell Reports. 2018 Jun 5;10(6):1766-1781. doi: 10.1016/j.stemcr.2018.03.015. Epub 2018 Apr 19.

NF-κB Activity Initiates Human ESC-Derived Neural Progenitor Cell Differentiation by Inducing a Metabolic Maturation Program.

Author information

1
School of Healthcare Sciences, Manchester Metropolitan University, Manchester M1 5GD, UK; Stem Cell Group, Cardiovascular & Cell Sciences Research Institute, St. George's University of London, Cranmer Terrace, London SW17 0RE, UK. Electronic address: l.fitzpatrick@mmu.ac.uk.
2
Stem Cell Group, Cardiovascular & Cell Sciences Research Institute, St. George's University of London, Cranmer Terrace, London SW17 0RE, UK.
3
Institute of Functional Biology and Genomics, University of Salamanca-CSIC, 37007 Salamanca, Spain; Institute of Biomedical Research of Salamanca, University Hospital of Salamanca, 37007 Salamanca, Spain.
4
Telethon Institute of Genetics and Medicine, Via Campi Flegrei 34, Pozzuoli, Naples 80078, Italy.
5
School of Healthcare Sciences, Manchester Metropolitan University, Manchester M1 5GD, UK.
6
Gene Transfer Technology Group, Institute for Women's Health, University College London, 86-96 Chenies Mews, London WC1E 6HX, UK; MRC Antiviral Gene Therapy Research Unit, Faculty of Health Sciences, University of Witswatersrand, Johannesburg, South Africa.
7
School of Healthcare Sciences, Manchester Metropolitan University, Manchester M1 5GD, UK; Stem Cell Group, Cardiovascular & Cell Sciences Research Institute, St. George's University of London, Cranmer Terrace, London SW17 0RE, UK.

Abstract

Human neural development begins at embryonic day 19 and marks the beginning of organogenesis. Neural stem cells in the neural tube undergo profound functional, morphological, and metabolic changes during neural specification, coordinated by a combination of exogenous and endogenous cues. The temporal cell signaling activities that mediate this process, during development and in the postnatal brain, are incompletely understood. We have applied gene expression studies and transcription factor-activated reporter lentiviruses during in vitro neural specification of human pluripotent stem cells. We show that nuclear factor κB orchestrates a multi-faceted metabolic program necessary for the maturation of neural progenitor cells during neurogenesis.

KEYWORDS:

NF-κB signaling; autophagy; cell cycle; human embryonic development; metabolism; neurogenesis

PMID:
29681545
PMCID:
PMC5989595
DOI:
10.1016/j.stemcr.2018.03.015
[Indexed for MEDLINE]
Free PMC Article

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