Format

Send to

Choose Destination
Mar Pollut Bull. 2018 Apr;129(1):61-69. doi: 10.1016/j.marpolbul.2018.02.015. Epub 2018 Feb 12.

Novel methodology to isolate microplastics from vegetal-rich samples.

Author information

1
Marine Ecophysiology Group (EOMAR), Iu-ECOAQUA, Universidad de Las Palmas de Gran Canaria, 35017 Campus Universitario de Tafira, Canary Islands, Spain. Electronic address: alicia.herrera@ulpgc.es.
2
Marine Ecophysiology Group (EOMAR), Iu-ECOAQUA, Universidad de Las Palmas de Gran Canaria, 35017 Campus Universitario de Tafira, Canary Islands, Spain.
3
Instituto de Tecnología de Materiales (ITM), Universitat Politècnica de València (UPV), Plaza Ferrándiz y Carbonell s/n, 03801 Alcoy, Alicante, Spain.

Abstract

Microplastics are small plastic particles, globally distributed throughout the oceans. To properly study them, all the methodologies for their sampling, extraction, and measurement should be standardized. For heterogeneous samples containing sediments, animal tissues and zooplankton, several procedures have been described. However, definitive methodologies for samples, rich in algae and plant material, have not yet been developed. The aim of this study was to find the best extraction protocol for vegetal-rich samples by comparing the efficacies of five previously described digestion methods, and a novel density separation method. A protocol using 96% ethanol for density separation was better than the five digestion methods tested, even better than using H2O2 digestion. As it was the most efficient, simple, safe and inexpensive method for isolating microplastics from vegetal rich samples, we recommend it as a standard separation method.

KEYWORDS:

Beach; Density separation; Marine litter; Microplastics; Organic material; Plastic extraction

PMID:
29680568
DOI:
10.1016/j.marpolbul.2018.02.015
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center