Format

Send to

Choose Destination
Int Immunopharmacol. 2018 Jun;59:339-346. doi: 10.1016/j.intimp.2018.03.034. Epub 2018 Apr 19.

Anti-inflammatory effect of Apo-9'-fucoxanthinone via inhibition of MAPKs and NF-kB signaling pathway in LPS-stimulated RAW 264.7 macrophages and zebrafish model.

Author information

1
Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology (KIOST), Jeju 63349, Republic of Korea.
2
Department of Marine Life Sciences, Jeju National University, Jeju 63243, Republic of Korea.
3
Marine-Integrated Bionics Research Center, Pukyong National University, Busan 48513, Republic of Korea.
4
Chuncheon Center, Korea Basic Science Institute (KBSI), Gangwon-do 24341, Republic of Korea.
5
Department of Microbiology, College of Medicine, Inje University, Busan 47392, Republic of Korea.
6
Jeju International Marine Science Center for Research & Education, Korea Institute of Ocean Science & Technology (KIOST), Jeju 63349, Republic of Korea; Department of Marine Biology, University of Science and Technology, Daejeon 34113, Republic of Korea. Electronic address: sjheo@kiost.ac.kr.

Abstract

In this study, we confirmed the anti-inflammatory effect of Apo-9-fucoxanthinone (AF) in in vitro RAW 264.7 cells and in vivo zebrafish model. In lipopolysaccharide (LPS)-stimulated zebrafish, AF significantly decreased the production of reactive oxygen species (ROS), nitric oxide (NO) and cell death. In addition, the mRNA expression of inducible nitric oxide synthase (iNOS), suppressed cyclooxygenase-2 (COX-2) and an inflammatory cytokines; IL-1β, TNF-α were shown reduction. And AF significantly inhibited NO production and expression of iNOS in LPS-stimulated RAW 264.7 cells. Further, AF suppressed COX-2, prostaglandin E2 (PGE2), and pro-inflammatory cytokines such as interleukin-6 (IL-6), IL-1β, and tumor necrosis factor-α (TNF-α) at 25, 50 and 100 μg/mL, respectively. Further mechanistic studies showed that AF suppressed the nuclear factor-kB (NF-kB) pathway and phosphorylation of mitogen-activated protein kinase (MAPK) pathway molecules such as extracellular signal-regulated kinase (ERK) and c-Jun N-terminal kinase (JNK). According to the results, AF can be used and applied as a useful anti-inflammatory agent of nutraceutical or pharmaceutical.

KEYWORDS:

Anti-inflammation; Apo-9′-fucoxanthinone; Cytokines; Macrophage; Seaweed; Zebrafish

PMID:
29679858
DOI:
10.1016/j.intimp.2018.03.034
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center