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Clin Biochem. 2018 Jun;56:95-101. doi: 10.1016/j.clinbiochem.2018.04.017. Epub 2018 Apr 19.

Serum retinol binding protein 4 and galectin-3 binding protein as novel markers for postmenopausal nonalcoholic fatty liver disease.

Author information

1
Affiliated Hangzhou People's Hospital of Nanjing Medical University, Hangzhou, Zhejiang, China.
2
Affiliated Hangzhou People's Hospital of Nanjing Medical University, Hangzhou, Zhejiang, China; Hangzhou Women's Hospital (Hangzhou Maternity and Child Health Care Hospital), Hangzhou, Zhejiang, China.
3
North Middlesex University Hospital NHS TRUST, London, UK.
4
Affiliated Hangzhou People's Hospital of Nanjing Medical University, Hangzhou, Zhejiang, China; Hangzhou Women's Hospital (Hangzhou Maternity and Child Health Care Hospital), Hangzhou, Zhejiang, China. Electronic address: zhangzf@zju.edu.cn.

Abstract

OBJECTIVE:

To investigate the differential protein expression before and after menopause in women with nonalcoholic fatty liver disease (NAFLD) and to explore novel markers for menopausal NAFLD.

METHODS:

Eight serum samples collected from pre- or post-menopausal women with NAFLD were analysed by iTRAQ 2D-LC-MS/MS. Two protein candidates were selected and verified by enzyme-linked immunosorbent assay (ELISA) in serum samples collected from a one hundred and fifty-three female population subsequently, including 51 in post-menopausal status with NAFLD, 41 in pre-menopausal with NAFLD, 19 healthy individuals in post-menopausal status and 42 healthy pre-menopausal women.

RESULTS:

A total of one hundred and sixty-seven proteins exhibiting significant changes were characterized, among which sixty-five were up-regulated and one hundred and two were down-regulated. Of those altered proteins, the expression of serum retinol binding protein 4 (RBP4) and galectin-3 binding protein (LGALS3BP) was obviously increased in the post-menopausal patient group compared to the other. ELISA validations for the two proteins were consistent with the proteomic profiling.

CONCLUSIONS:

Serum RBP4 and LGAL3BP were up-regulated after menopause under NAFLD conditions, which suggested the two proteins may be potential markers as NAFLD in postmenopausal population.

KEYWORDS:

Galectin-3 binding protein; Menopause; Nonalcoholic fatty liver disease; Proteomics; Serum retinol binding protein 4

[Indexed for MEDLINE]

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