The Relationship of Methylenetetrahydrofolate Reductase Gene C677T Polymorphism and Ischemic Stroke in Chinese Han Population

Ann Clin Lab Sci. 2018 Mar;48(2):242-247.

Abstract

Background: The purpose of this study was to investigate the relationship of methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphism and ischemic stroke Large-artery atherosclerosis (LAA) and Small-artery occlusion (SAO) subtypes in Chinese Han Population.

Materials and methods: One hundred and thirty LAA patients and one hundred and fifty-nine SAO patients data were prospectively collected. The control group was one hundred and ninety-eight subjects from the Medical Examination Center in the same period. Plasma homocysteine (Hcy) levels were determined by a double reagent enzymatic cycling method. The genotypes of MTHFR polymorphism (C677T) were determined using polymerase chain reaction-restriction fragment length polymorphism.

Results: As compared with the controls, hypertension prevalence, smoking consumption ratio were significantly higher, plasma Hcy levels increased significantly, plasma high-density lipoprotein cholesterol levels were significantly reduced in LAA and SAO groups (P<0.05 for each). Elevated plasma Hcy levels were associated with MTHFR C677T polymorphism (P<0.01). The frequency of C/T+T/T genotypes and T allele in LAA and SAO groups was significantly higher than those in control groups (P<0.01).

Conclusions: The present study suggests that hypertension, smoking, decreasing plasma high-density lipoprotein cholesterol levels and hyperhomocysteinemia (HHcy) are independent risk factors for LAA and SAO subtypes of ischemic stroke in Chinese Han Population. The MTHFR gene C677T C/T+T/T genotypes and T allele increases the risk of LAA and SAO subtypes of ischemic stroke.

Keywords: Ischemic stroke; gene polymorphism; homocysteine; methylenetetrahydrofolate reductase.

MeSH terms

  • Aged
  • Asian People
  • Brain Ischemia / complications
  • China
  • Female
  • Gene Frequency
  • Genetic Predisposition to Disease / genetics*
  • Genotype
  • Homocysteine / blood
  • Humans
  • Logistic Models
  • Male
  • Methylenetetrahydrofolate Reductase (NADPH2) / genetics*
  • Middle Aged
  • Polymorphism, Genetic / genetics*
  • Risk Factors
  • Statistics, Nonparametric
  • Stroke / blood
  • Stroke / etiology
  • Stroke / genetics*

Substances

  • Homocysteine
  • Methylenetetrahydrofolate Reductase (NADPH2)