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Electrophoresis. 2018 Jun;39(12):1488-1496. doi: 10.1002/elps.201700377. Epub 2018 May 16.

Optimized two-dimensional gel electrophoresis in an alkaline pH range improves the identification of intracellular CFDA-cisplatin-protein adducts in ovarian cancer cells.

Author information

1
MS-Platform Biocenter, Cluster of Excellence on Plant Science (CEPLAS), University of Cologne, Cologne, Germany.
2
IUF-Leibniz Research Institute for Environmental Medicine, Heisenberg-group - Environmentally-induced cardiovascular degeneration, Mass spectrometry Core Unit, Düsseldorf, Germany.
3
Department of Clinical Pharmacy, Institute of Pharmacy, University of Bonn, Bonn, Germany.

Abstract

Intracellular binding of cisplatin to proteins has been associated with acquired resistance to chemotherapy. In our previous study we established an analytical method for the identification of intracellular cisplatin-binding proteins. The method used a fluorescent carboxyfluorescein-diacetate-labeled cisplatin analogue (CFDA-cisplatin), two-dimensional gel electrophoresis (2DE) and mass spectrometry, which allows detecting and identifying intracellular CFDA-cisplatin-containing protein adducts in the acidic pH range (pH 4-7). Based on this analytical method we extended the identification of intracellular cisplatin-protein adducts to the alkaline pH range (pH 6-10) giving chance to discover new important binding partners. 2DE analysis of alkaline proteins is challenging due to the difficult separation of basic proteins during the isoelectric focusing (IEF). The establishment of an optimized IEF protocol for basic proteins enabled us to identify several intracellular CFDA-cisplatin-binding proteins including enzymes of the glucose and serine metabolism like alpha enolase and D-3-phosphoglycerate 1-dehydrogenase.

KEYWORDS:

Alkaline pH-range; CFDA-cisplatin; Cisplatin; Two-dimensional gel electrophoresis

PMID:
29676816
DOI:
10.1002/elps.201700377

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