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Nat Commun. 2018 Apr 19;9(1):1561. doi: 10.1038/s41467-018-03899-1.

Gold-nanofève surface-enhanced Raman spectroscopy visualizes hypotaurine as a robust anti-oxidant consumed in cancer survival.

Author information

1
Frontier Core-Technology Laboratories, Research & Development Management Headquarters, FUJIFILM Corporation, 577, Ushijima, Kaisei-machi, Ashigarakami-gun, Kanagawa, 258-8577, Japan.
2
Department of Biochemistry, Keio University School of Medicine, Tokyo, 160-8582, Japan.
3
Analysis Technology Center, Research & Development Management Headquarters, FUJIFILM Corporation, 577, Ushijima, Kaisei-machi, Ashigarakami-gun, Kanagawa, 258-8577, Japan.
4
Functional Materials R&D Support Department, Research & Development Process Services Division, FUJIFILM Business Expert Corporation, 577, Ushijima, Kaisei-machi, Ashigarakami-gun, Kanagawa, 258-8577, Japan.
5
Pathology Division, National Cancer Center Research Institute, Tokyo, 104-0045, Japan.
6
Department of Stem Cell Biology, Research Institute, National Center for Global Health and Medicine, Tokyo, 162-8655, Japan.
7
Division of Gene Regulation, Institute for Advanced Medical Research, Keio University School of Medicine, Tokyo, 160-8582, Japan.
8
Department of Biochemistry, Keio University School of Medicine, Tokyo, 160-8582, Japan. gasbiology@keio.jp.

Abstract

Gold deposition with diagonal angle towards boehmite-based nanostructure creates random arrays of horse-bean-shaped nanostructures named gold-nanofève (GNF). GNF generates many electromagnetic hotspots as surface-enhanced Raman spectroscopy (SERS) excitation sources, and enables large-area visualization of molecular vibration fingerprints of metabolites in human cancer xenografts in livers of immunodeficient mice with sufficient sensitivity and uniformity. Differential screening of GNF-SERS signals in tumours and those in parenchyma demarcated tumour boundaries in liver tissues. Furthermore, GNF-SERS combined with quantum chemical calculation identified cysteine-derived glutathione and hypotaurine (HT) as tumour-dominant and parenchyma-dominant metabolites, respectively. CD44 knockdown in cancer diminished glutathione, but not HT in tumours. Mechanisms whereby tumours sustained HT under CD44-knockdown conditions include upregulation of PHGDH, PSAT1 and PSPH that drove glycolysis-dependent activation of serine/glycine-cleavage systems to provide one-methyl group for HT synthesis. HT was rapidly converted into taurine in cancer cells, suggesting that HT is a robust anti-oxidant for their survival under glutathione-suppressed conditions.

PMID:
29674746
PMCID:
PMC5908798
DOI:
10.1038/s41467-018-03899-1
[Indexed for MEDLINE]
Free PMC Article

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