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J Proteomics. 2018 Jun 15;181:170-189. doi: 10.1016/j.jprot.2018.04.017. Epub 2018 Apr 17.

Novel functionalized nanomaterials for the effective enrichment of proteins and peptides with post-translational modifications.

Author information

1
Research Unit, Hospital Universitario Lucus Augusti (HULA), Servizo Galego de Saúde (SERGAS), 27002 Lugo, Spain. Electronic address: cristina.nunez.gonzalez@sergas.es.
2
Research Unit, Hospital Universitario Lucus Augusti (HULA), Servizo Galego de Saúde (SERGAS), 27002 Lugo, Spain.
3
Proteomic Unit, Instituto de Investigaciones Sanitarias-IDIS, Complejo Hospitalario Universitario de Santiago de Compostela (CHUS), 15706 Santiago de Compostela, Spain.
4
Oncology Division, Hospital Universitario Lucus Augusti (HULA), Servizo Galego de Saúde (SERGAS), 27002 Lugo, Spain.

Abstract

Low abundance proteins/peptides usually suffer strong interference with highly abundant proteins/peptides as well as other contaminants, resulting in low ionization efficiency in MS analysis. Hence, the enrichment and separation of proteins/peptides from complex mixtures are of great value to their successful identification. In this review we present an overview of the application of different nanocomposites for the specific enrichment of peptides/proteins with post-translational modifications (PTMs), such as phosphorylation and glycosylation. Furthermore, examples of the selective enrichment of cysteine-containing peptides, histidine-tagged proteins and the isolation of N-terminal peptides will be analyzed.

KEYWORDS:

Sample pretreatment; enrichment; glycoproteins/glycopeptides; low-abundance proteins/peptides; nanomaterial; phosphoproteins/phosphopetides

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