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Digestion. 2018;98(1):56-68. doi: 10.1159/000487186. Epub 2018 Apr 19.

Modulation of Pacemaker Potentials in Murine Small Intestinal Interstitial Cells of Cajal by Gamisoyo-San, a Traditional Chinese Herbal Medicine.

Author information

1
Division of Longevity and Biofunctional Medicine, Yangsan, Republic of Korea.
2
Healthy Aging Korean Medical Research Center (HAKMRC), Pusan National University School of Korean Medicine, Yangsan, Republic of Korea.
3
Department of Physiology, College of Medicine, Dongguk University, Kyungju, Republic of Korea.
4
Department of Pharmaceutical Engineering, Daegu Haany University, Gyeongsan, Republic of Korea.
5
College of Oriental Medicine, Daegu Haany University, Gyeongsan, Republic of Korea.

Abstract

BACKGROUND:

The Gamisoyo-san (GSS) has been used for -improving the gastrointestinal (GI) symptoms. The purpose of this study was to investigate the effects of GSS, a traditional Chinese herbal medicine, on the pacemaker potentials of mouse small intestinal interstitial cells of Cajal (ICCs).

METHODS:

ICCs from the small intestines were dissociated and cultured. Whole-cell patch-clamp configuration was used to record pacemaker potentials and membrane currents.

RESULTS:

GSS depolarized ICC pacemaker potentials in a dose-dependent manner. Pretreatment with 4-diphenylacetoxypiperidinium iodide completely inhibited GSS-induced pacemaker potential depolarizations. Intracellular GDP-β-S inhibited GSS-induced effects, and in the presence of U-73122, GSS-induced effects were inhibited. Also, GSS in the presence of a Ca2+-free solution or thapsigargin did not depolarize pacemaker potentials. However, in the presence of calphostin C, GSS slightly depolarized pacemaker potentials. Furthermore, GSS inhibited both transient receptor potential melastatin7 and Ca2+-activated Cl- channel (anoctamin1) currents.

CONCLUSION:

GSS depolarized pacemaker potentials of ICCs via G protein and muscarinic M3 receptor signaling pathways and through internal or external Ca2+-, phospholipase C-, and protein kinase C-dependent and transient receptor potential melastatin 7-, and anoctamin 1-independent pathways. The study shows that GSS may regulate GI tract motility, suggesting that GSS could be a basis for developing novel prokinetic agents for treating GI motility dysfunctions.

KEYWORDS:

Gamisoyo-San; Gastrointestinal motility; Gastrointestinal tract; Interstitial cells of Cajal; Prokinetic agent

PMID:
29672308
DOI:
10.1159/000487186
[Indexed for MEDLINE]

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