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J Endocrinol Invest. 2019 Jan;42(1):27-35. doi: 10.1007/s40618-018-0877-1. Epub 2018 Apr 18.

Effects of growth hormone on pregnancy rates of patients with thin endometrium.

Author information

1
Department of Reproductive Medicine, The Second Hospital, Hebei Medical University, 215 West Heping Road, Shijiazhuang, 050000, Hebei Province, People's Republic of China.
2
Department of Reproductive Medicine, The Second Hospital, Hebei Medical University, 215 West Heping Road, Shijiazhuang, 050000, Hebei Province, People's Republic of China. haoguimin@163.com.
3
Department of Interventional Therapy, Henan Provincial People's Hospital, 7 Weiwu Road, Zhengzhou, 450003, Hebei Province, People's Republic of China. browngao@163.com.

Abstract

PURPOSE:

To investigate whether growth hormone (GH) could improve pregnancy rates of patients with thin endometrium by clinical study and laboratory experiments.

MATERIALS AND METHODS:

Ninety-three patients were randomized to either the GH-received group (40) or the routine exogenous administration of estrogens control group (53) for clinical study. The human endometrial carcinoma cell line RL95-2 was used for testing the role of GH with Western blot and real-time PCR by exposure to various concentrations of GH (0.1 nM,1 nM,10 nM,100 nM).

RESULTS:

Patients treated with GH had a significantly (P < 0.05) greater endometrium thickness on day 3 (7.87±0.72 vs 6.34±0.86), higher implantation rates (24.4% vs 10.5%) and greater clinical pregnancy rates (42.5% vs 18.9%) compared with the control group. No adverse events were associated with the use of GH. Administration of GH significantly up-regulated the expression of VEGF, ItgB3 and IGF-I expression in RL95-2 cells at both mRNA and protein levels (P < 0.05). AG490, an inhibitor of JAK2, nearly completely inhibited the up-regulative effect of GH through the JAK2-STAT5 pathway, and GH-induced effects could be mediated through autocrine IGF-I together with its hepatic counterpart. IGF-I mRNA was detected in the RL95-2 cells.

CONCLUSION:

GH may improve pregnancy outcomes of patients with thin endometrium who undergo frozen embryo transfer by acting on human endometrial cells to promote proliferation and vascularization and to up-regulate receptivity-related molecular expression.

KEYWORDS:

Cell experiment; Endometrial thickness; Growth hormone; Mechanism; Pregnancy rate

PMID:
29671256
DOI:
10.1007/s40618-018-0877-1
[Indexed for MEDLINE]

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