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Molecules. 2018 Apr 18;23(4). pii: E936. doi: 10.3390/molecules23040936.

Fluorescent Dendritic Micro-Hydrogels: Synthesis, Analysis and Use in Single-Cell Detection.

Author information

1
Department of Chemistry, Boston University, Boston, MA 02215, USA. lmchristadore1@gmail.com.
2
Department of Chemistry, Boston University, Boston, MA 02215, USA. mgrin@bu.edu.
3
Departments of Biomedical Engineering and Medicine, Boston University, Boston, MA 02215, USA. mgrin@bu.edu.
4
Department of Chemistry, Boston University, Boston, MA 02215, USA. seschaus@bu.edu.

Abstract

Hydrogels are of keen interest for a wide range of medical and biotechnological applications including as 3D substrate structures for the detection of proteins, nucleic acids, and cells. Hydrogel parameters such as polymer wt % and crosslink density are typically altered for a specific application; now, fluorescence can be incorporated into such criteria by specific macromonomer selection. Intrinsic fluorescence was observed at λmax 445 nm from hydrogels polymerized from lysine and aldehyde- terminated poly(ethylene glycol) macromonomers upon excitation with visible light. The hydrogel’s photochemical properties are consistent with formation of a nitrone functionality. Printed hydrogels of 150 μm were used to detect individual cell adherence via a decreased in fluorescence. The use of such intrinsically fluorescent hydrogels as a platform for cell sorting and detection expands the current repertoire of tools available.

KEYWORDS:

crosslinking; dendrimer; fluorescence; hydrogel; screening

PMID:
29669998
PMCID:
PMC6017717
DOI:
10.3390/molecules23040936
[Indexed for MEDLINE]
Free PMC Article

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