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Nutr Cancer. 2018 May-Jun;70(4):605-619. doi: 10.1080/01635581.2018.1460673. Epub 2018 Apr 18.

Antiproliferative Effect of Vine Stem Extract from Spatholobus Suberectus Dunn on Rat C6 Glioma Cells Through Regulation of ROS, Mitochondrial Depolarization, and P21 Protein Expression.

Author information

1
a Department of Biotechnology , Hoseo University , Asan , Chungnam , Republic of Korea.
2
b Department of Biomedical Laboratory Science , College of Biomedical Sciences, Soonchunhyang University , Asan , Chungnam , Republic of Korea.
3
c Department of Animal Biotechnology , Chonbuk National University , Jeonju , Jeonbuk , Republic of Korea.
4
d Lab of Immunology, Integrated Research Institute of Pharmaceutical Sciences, College of Pharmacy, The Catholic University of Korea , Bucheon , Gyeonggi , Republic of Korea.
5
e Department of Medical Biotechnology , College of Medical Sciences, Soonchunhyang University , Asan , Chungnam , Republic of Korea.
6
f Department of Biotechnology and Bioinformatics , College of Science and Technology, Korea University , Sejong , Republic of Korea.

Abstract

The vine stem of Spatholobus suberectus Dunn (SS) is used as a traditional herbal medicine in China. Chinese herbal medicines are well known as natural bioactive compounds that can be used as new medicines, and their antioxidant and anticancer effects have also been reported. This study aimed to examine the anticancer effect of a high-pressure hot-water SS extract on rat C6 glioma cells. The SS extract effectively suppressed the viability and proliferation of C6 glioma cells through an antioxidant effect. Reactive oxygen species (ROS) levels in cancer cells are higher than that in normal cells. If the ROS level falls below that required for the growth of cancer cells, their rapid proliferation and growth can be suppressed. We also measured the induction of mitochondrial membrane depolarization and cell cycle arrest effect caused by the SS extract in C6 glioma cells through a FACS analysis. In addition, we observed an increase in STAT3, p53, E2F1, and p21 mRNA expression and a decrease in Bcl-2 mRNA expression by quantitative PCR. An increase in p21 protein expression of over 83% was observed through western blot analysis. All these data support the fact that the high-pressure hot-water SS extract has the potential to be used for glioma treatment.

PMID:
29668336
DOI:
10.1080/01635581.2018.1460673
[Indexed for MEDLINE]

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