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Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2018 Apr;26(2):563-568. doi: 10.7534/j.issn.1009-2137.2018.02.043.

[Effect of MiR-99a-5p on the Differential Ability of Human Bone Marrow Mesenchymal Stem Cells].

[Article in Chinese]

Author information

1
Department of Hematology, Anhui Provincial Hospital,Affiliated to Anhui Medical University, Hefei 230001, Anhui Province, China.
2
Department of Hematology, Anhui Provincial Hospital,Affiliated to Anhui Medical University, Hefei 230001, Anhui Province, China. E-mail:Wangxingbing91@hotmail.com.

Abstract

OBJECTIVE:

To investigate the effect of microRNA-99a-5p (miR-99a-5p) on differentiation ability of human bone marrow mesenchymal stem cells (BM-MSC).

METHODS:

BM-MSC was cultured and then transfected with miR-99a-5p mimics or inhibitors. The transfection efficiency was detected by real-time quantitative PCR. The effects of miR-99a-5p on the adipogenic and osteogenic differentiation ability of BM-MSC were detected by differentiation experiment.

RESULTS:

As compared with the negative control group, the expression of miR-99a-5p was significantly up-regulated after transfection with miR-99a-5p mimics(P<0.001), the expression of miR-99a-5p was down-regulated after transfection with miR-99a-5p inhibitor (P<0.001). In osteogenic differentiation experiments, the miR-99a-5p overexpression could promote the osteogenic differentiation, while the downregulation of miR-99a-5p expression inhibited the osteogenic differentiation. The same results were obtained by semi-quantitative detection through spectrophotometry. In the adipogenic differentiation test, transfection of miR-99a-5p mimics or inhibitors had no significant effect on the adipogenic differentiation of BM-MSC.

CONCLUSION:

Overexpression of miR-99a-5p can promote the osteogenic differentiation of BM-MSC, but no significant effects are observed in the adipogenic differentiation.

[Indexed for MEDLINE]

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