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Malar J. 2018 Apr 17;17(1):169. doi: 10.1186/s12936-018-2257-x.

Elevated IL-17 levels in semi-immune anaemic mice infected with Plasmodium berghei ANKA.

Author information

1
Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.
2
Department of Biochemistry and Molecular Medicine, School of Medicine and Health Sciences, University for Development Studies, Tamale, Ghana.
3
West Africa Center for Cell Biology of Infectious Pathogens (WACCBIP), Department of Biochemistry, Cell and Molecular Biology, University of Ghana, Accra, Ghana.
4
National Bio-Resource Center (NBRC), NEKKEN, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan.
5
Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria.
6
Department of Immunogenetics, Institute of Tropical Medicine (NEKKEN), Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan. hiraken@nagasaki-u.ac.jp.
7
National Bio-Resource Center (NBRC), NEKKEN, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, 852-8523, Japan. hiraken@nagasaki-u.ac.jp.

Abstract

BACKGROUND:

Alterations in inflammatory cytokines and genetic background of the host contribute to the outcome of malaria infection. Despite the promising protective role of IL-17 in infections, little attention is given to further understand its importance in the pathogenesis of severe malaria anaemia in chronic/endemic situations. The objective of this study, therefore, was to evaluate IL-17 levels in anaemic condition and its association with host genetic factors.

METHODS:

Two mice strains (Balb/c and CBA) were crossed to get the F1 progeny, and were (F1, Balb/c, CBA) taken through 6 cycles of Plasmodium berghei (ANKA strain) infection and chloroquine/pyrimethamine treatment to generate semi-immune status. Cytokine levels and kinetics of antibody production, CD4+CD25+T regulatory cells were evaluated by bead-based multiplex assay kit, ELISA and FACs, respectively.

RESULTS:

High survival with high Hb loss at significantly low parasitaemia was observed in Balb/c and F1. Furthermore, IgG levels were two times higher in Balb/c, F1 than CBA. While CD4+CD25+ Treg cells were lower in CBA; IL-4, IFN-γ, IL-12α and IL-17 were significantly higher (p < 0.05) in Balb/c, F1.

CONCLUSIONS:

In conclusion, elevated IL-17 levels together with high IL-4, IL-12α and IFN-γ levels may be a marker of protection, and the mechanism may be controlled by host factor (s). Further studies of F2 between the F1 and Balb/c will be informative in evaluating if these genes are segregated or further apart.

KEYWORDS:

Anaemia; IL-17; Plasmodium berghei ANKA; Semi-immune mice

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