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Arch Physiol Biochem. 2019 Jul;125(3):270-275. doi: 10.1080/13813455.2018.1455708. Epub 2018 Apr 17.

Therapeutic role of calcium and vitamin K3 in chemically induced hepatocarcinogenesis - new tools for cancer treatment.

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a Department of Biochemistry , Faculty of Science, King Abdulaziz University , Jeddah , Saudi Arabia.
b Siddhartha Institute of Pharmacy , Dehradun , India.
c School of Pharmacy, Sungkyunkwan University , Republic of Korea.
d Glocal School of Pharmacy, Glocal University , Saharanpur , Uttar Pradesh, India.
e Department of Pharmacology , Jouf University , Sakaka , Kingdom of Saudi Arabia.
f Centre for Advanced Research in Pharmaceutical Sciences, Microbial and Pharmaceutical Biotechnology Laboratory, Faculty of Pharmacy , JamiaHamdard , New Delhi, India.
g Natural Product Drug Discovery Laboratory, Department of Pharmaceutical Sciences, Shalom Institute of Health & Allied Sciences , Sam Higginbottom University of Agriculture, Technology & Sciences (SHUATS) , Allahabad , Uttar Pradesh, India.


HCC has been reported to be immensely occurring carcinoma worldwide. Recent days the mortality occurred due to liver cancer has also been found to be increased at an alarming speed affecting mostly the young patients. The aim of the current study was to decipher the role of calcium and vitamin K3 in the treatment of chemically induced hepatocarcinogenesis in the male Wistar rats. Liver cancer was induced via a subnecrogenic dose of 160 mg/kg body weight, diethylnitrosamine (DENA) when associated with fasting/refeeding in male Wistar rats. It elevated the serum glutamate oxaloacetate (SGOT), serum glutamate pyruvate transaminase (SGPT), alkaline phosphatase (ALP), bilirubin, total cholesterol (CH), triglycerides (TG), alfa-fetoprotein (AFP) and reduced high-density lipoprotein (HDL). Histopathological examination of liver tissue showed marked carcinogenicity of the chemical carcinogen. Food, water intake and animal weights were also assessed, respectively. The animals exposed to DENA showed a significant decrease in the body weight. The elevated levels of serum SGOT, SGPT, ALP, AFP, TC and TG were restored by administration of calcium and Vit K (ad libitum) combination at higher dose than the normal dietary requirement (3 mg/kg) daily for 12 weeks p.o. Physiological and biochemical analysis showed the beneficial effects of calcium and vitamin K3 combination in the animals exposed to DENA. The results deciphered the beneficial effects of calcium and vitamin K3 in combination.


DENA; anticancer; calcium; hepatocellular carcinoma; vitamin K

[Indexed for MEDLINE]

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