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Arch Physiol Biochem. 2018 Apr 17:1-12. doi: 10.1080/13813455.2018.1461233. [Epub ahead of print]

Antioxidant and hepato-preventive effect of Citrus aurantium extract against carbon tetrachloride-induced hepatotoxicity in rats and characterisation of its bioactive compounds by HPLC-MS.

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a Biotechnology and Plant Improvement Laboratory , Centre of Biotechnology of Sfax , Sfax , Tunisia.
b Laboratory of Animal Ecophysiology, Faculty of Sciences , University of Sfax , Sfax , Tunisia.
c Laboratory of Biotechnology and Valorisation of Bio-GeoRessources (BVBGR), Higher Institute of Biotechnology of Sidi Thabet (ISBST) , University of Manouba, Biotechpole Sidi Thabet , Ariana , Tunisia.


The objective of this study is to explore the preventive effects of ethyl acetate fraction from Citrus aurantium leaf extract (EACA), associated with its phytochemical content, against the toxic impacts of acute exposure to carbon tetrachloride (CCl4) in the liver of adult rats. HPLC analysis of ethyl acetate fraction from extract C. aurantium revealed eight compounds. Administration of a single dose of CCl4 caused hepatoxicity as monitored by an increase in lipid peroxidation (thiobarbituric acid reactive substances) and in protein carbonyl level but a decrease in antioxidant markers in the liver tissue. The pre-treatment with EACA; significantly prevented the increased plasma levels of hepatic markers and lipid levels induced by CCl4 in rats. Furthermore, this fraction ameliorated biochemical and histological parameters as compared to CCl4-treated group. Our results suggest that C. aurantium contains promising substances to counteract the CCl4 intoxication and can be efficient in the prevention of hepatotoxicity complications.


Carbon tetrachloride; Citrus aurantium; HPLC-MS; extract fractionation; hepato-preventive; oxidative stress; phytochemical; rats

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